Ketogulonicigenium

Ascorbic acid Ketogulonicigenium vulgare Xanthomonas campestris... 

Elucidation of the Mechanisms in Mutualism Between Bacillus megaterium and Ketogulonicigenium vulgare... 

Sugisawa T, Miyazaki T, Hoshino T (2005) Microbial production of L-ascorbic acid from D-sorbitol, L-sotbose, L-gulose, and L-sotbosone by Ketogulonicigenium vulgare DSM 4025. Biosd Biotechnol Biochem 69 659-662... 

During the last four decades many attempts have been made to discover improved microbial methods for the production of 2-KGA from various substrates, including D-glucose, D-sorbitol, L-sorbose, L-sorbosone and L-iodonic acid (for chemical structures, see Figs. 16.2 and 16.3). The fist of employed micro-orgaifisms consists of Gluconobacter, Ketogulonicigenium, Erwinia, Corynebacterium and Pseudomonas strains. 

At DSM Nutritional Products Ltd the main research activities were focused on processes employing Gluconobacter and Ketogulonicigenium strains. In 1990, an improved 2-KGA production was described with mutants of Gluconobacter melanogenus starting from D-sorbitol or L-sorbose. " With a yield on substrates of 60%, a final 2-KGA concentration of 60 g/L was achieved within 90 h of cultivation. 

Numerous attempts have been made to exploit new routes for the direct production of L-ascorbic acid from inexpensive feedstocks in a commercially attractive way. The list of putative production organisms contains microalgae (Chlorella, Prototheca), yeasts Candida, Saccharomyces, Zygosaccharomyces), and (recombinant) prokaryotes (Gluconobacter, Ketogulonicigenium, Xanthomonas). 

Takagi, Y., Sugisawa, T. and Hoshino, T. (2009). Continuous 2-keto-L-gulonic Acid Fermentation from L-sorbose by Ketogulonicigenium vulgare DSM 4025, Appl. Microbiol. BiotechnoL, 82, pp. 1049-1056. 

Zhou, J., Ma, Q., Yi, H., Wang, L.L. et al. (2011) Metabolome profiling reveals metabolic cooperation between Bacillus megaterium and Ketogulonicigenium vulgare during induced swarm motility. Appl. Environ. Microbiol, 77, 7023 -7030. 

H. et al. (2012) Reconstruction and analysis of a genome-scale metabolic model of the vitamin C producing industrial strain Ketogulonicigenium vulgare WSH-001. J. Biotechnol, 161, 42 -48. 

Gao, L.L., Du, G, Zhou, J., Chen, J. et al. (2013) Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-keto-L-gulonic acid in Ketogulonicigenium vulgare WSH-001. Biotechnol Progr, 29, 1398-1404. 

Miyazaki T, Sugisawa T, Hoshino T (2006) Pyrroloquinoline quinone-dependent dehydrogenases from Ketogulonicigenium vulgare catalyze the direct conversion of L-sorbosone to L-ascorbic acid. Appl Environ Microbiol 72 1487-1495... 

Chapter 15 Acetobacter aceti Komagataeibacter Gluconobacter Ketogulonicigenium... 

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