Kanamycin-neomycin acetyl transferase

Among the enzymes involved in the resistance mechanism, kanamycin-neomycin acetyl transferase was first found by Umezawa and coworkers in Escherichia coU K12 R5 carrying R factor. This strain was obtained by transmission of R factor from naturally isolated, drug-resistant, dysentery bacteria. 

Kanamycin-neomycin acetyl transferase. Kanamycin-neomycin phosphate... 

Escherichia coli R5 has a kanamydn-neomycin acetyl transferase that acetylates the 6 -amino group. However, resistance of this strain to kanamydns is very weak. Pseudomonas aeruginosa CN315 is highly resistant to these antibiotics here, 6 -N-methylation of 3, 4 -dideoxy-kanamycin B (Ref. 47) gives a compound that is active against this strain, as may be seen in Table III. 

Ps. aeruginosa Gentamicin acetyl-transferase I + Neomycin-kanamycin phosphotransferase 4 64 32... 

Kanamycin acetyl transferase was also found in Pseudomonas aeruginosa GN315, which was resistant to kanamycin, 3, 4 -dideoxykanamydn B, neomycins, and ribostamycin. ... 

The first experiments involving foreign gene expression in higher plants used the coding regions of the bacterial genes for chloramphenicol acetyl transferase (CAT) (47) and neomycin phosphotransferase (NPT-II) (48) fused to the nopallne synthase promoter (49,50). When neomycin phosphotransferase was expressed in tobacco, it was able to detoxify kanamycin, which normally kills plant cells (35,51-53). 

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