Isotope compound specific

Hydro-de-diazoniation seems to be an unnecessary reaction from the synthetic standpoint, as arenediazonium salts are obtained from the respective amines, reagents that are normally synthesized from the hydrocarbon. Some aromatic compounds, however, cannot be synthesized by straightforward electrophilic aromatic substitution examples of these are the 1,3,5-trichloro- and -tribromobenzenes (see below). These simple benzene derivatives are synthesized from aniline via halogenation, diazotization and hydro-de-diazoniation. Furthermore hydro-de-diazoniation is useful for the introduction of a hydrogen isotope in specific positions. 

Stott, A.W., Davies, E., Evershed, R.E. and Tuross, N. 1997 Monitoring the routing of dietary and biosynthesized lipids through compound-specific stable isotope (delta C) measurements at natural abundance. Naturwissenschcften 84(2) 82-86. 

Jarman WM, A Hilkert, CE Bacon, JW Collister, K Ballschmiter, RW Risebrough (1998) Compound-specific carbon isotopic analysis of Arochlors, Clophens, Kanechlors, and Phenoclors. Environ Sci Technol 32 833-836. 

Mass spectrometry can be specific in certain cases, and would even allow on-line QA in the isotope dilution mode. MS of molecular ions is seldom used in speciation analysis. API-MS allows compound-specific information to be obtained. APCI-MS offers the unique possibility of having an element- and compound-specific detector. A drawback is the limited sensitivity of APCI-MS in the element-specific detection mode. This can be overcome by use of on-line sample enrichment, e.g. SPE-HPLC-MS. The capabilities of ESI-MS for metal speciation have been critically assessed [546], Use of ESI-MS in metal speciation is growing. Houk [547] has emphasised that neither ICP-MS (elemental information) nor ESI-MS (molecular information) alone are adequate for identification of unknown elemental species at trace levels in complex mixtures. Consequently, a plea was made for simultaneous use of these two types of ion source on the same liquid chromatographic effluent. 

Compound-specific Stable Isotopes in Organic Residue Analysis in Archaeology... 

The aims of this contribution are to (i) consider the theoretical principles underlying the use of compound-specific stable isotope analysis in archaeology (ii) consider the practical aspects of undertaking compound-specific stable isotope analyses and (iii) demonstrate the value of linking the structures of amino acids, fatty acids and/or sterols, to their compound-specific stable isotope values to achieve new insights into variations in metabolism and environment in order to enhance archaeological interpretations. 

Compound-specific stable isotope analyses are more complex to undertake than bulk stable isotope analyses and require careful consideration to be given to sample... 

Figure 14.5 Schematic diagram of instrumental variants of stable isotope analysis providing either bulk or compound specific stable isotope values...

Figure 14.6 Generalised analytical protocol for the compound specific stable isotope analysis of free and building block components of complex archaeological materials...

Derivatisations for Compound-specific Stable Isotope Analysis... 

GC-C-IRMS instrumentation enables the compound-specific isotope analysis of individual organic compounds, for example, n-alkanes, fatty acids, sterols and amino acids, extracted and purified from bulk organic materials. The principle caveat of compound-specific work is the requirement for chemical modification, or derivatisation, of compounds containing polar functional groups primarily to enhance their volatility prior to introduction to the GC-C-IRMS instrument. Figure 14.7 summarises the most commonly employed procedures for derivatisation of polar, nonvolatile compounds for compound-specific stable isotope analysis using GC-C-IRMS. 

Instrumental Aspects of Compound-specific Stable Isotope Determinations... 

Compound-specific Stable Carbon Isotope Snalysis of Bone and Tissue Biochemical Components... 

Figure 14.12 Trends through time in the bulk stable isotope values of collagen (6I3C and 815N), apatite ( 13C) and compound specific 813C values of cholesterol recorded from a number of individuals from the Illinois River Valley, USA, showing contiguous fluctuations in carbon stable isotope composition consistent with differing contributions of maize to the diet (Jim, Ambrose and Evershed unpublished data)...

Compound-specific Carbon Isotope Values and the Origins of Animal Fats... 

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