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Isopropyl-/3-D-thiogalactopyranoside IPTG

Effect of the addition of isopropyl- 3-D-thiogalactopyranoside (IPTG) (USB) at set cell densities (OD660between0.01 and 0.8) corresponding to cultures at 103-107 CFU/mL. [Pg.455]

Concentrated PBS (10X), 0.5 Af ethylendiamine tetraacetic acid (EDTA), 1 Af Tris-HCl pH 8.0, 5 Af NaCl, 3 AfNaAc pH 5.2, kanamycin and 1 M isopropyl-(3-D-thiogalactopyranoside (IPTG) from Gibco/BRL (Bethesda, MD). [Pg.285]

GST-C3 fusion protein expression is induced by adding isopropyl- 3-D-thiogalactopyranoside (IPTG Calbiochem) to 0.2 mM (1 ml of 0.1 M stock made in water and stored at -20 "C), and the culture is incubated with shaking for a further 3h. [Pg.73]

See other pages where Isopropyl-/3-D-thiogalactopyranoside IPTG is mentioned: [Pg.291]    [Pg.115]    [Pg.210]    [Pg.164]    [Pg.155]    [Pg.402]    [Pg.219]    [Pg.302]    [Pg.291]    [Pg.115]    [Pg.210]    [Pg.164]    [Pg.155]    [Pg.402]    [Pg.219]    [Pg.302]    [Pg.421]    [Pg.409]    [Pg.88]    [Pg.327]    [Pg.165]    [Pg.36]    [Pg.193]    [Pg.70]    [Pg.433]    [Pg.92]    [Pg.248]    [Pg.19]    [Pg.253]    [Pg.92]    [Pg.59]    [Pg.127]    [Pg.393]    [Pg.54]    [Pg.162]    [Pg.305]   
See also in sourсe #XX -- [ Pg.93 ]



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Isopropyl-/3-D-thiogalactopyranoside

Thiogalactopyranosides

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