Isopropyl-/3-D-thiogalactopyranoside

The culture was charged with 1 mM isopropyl-/3-D-thiogalactopyranoside to induce gene expression and cultivated another 3 h. 

Effect of the addition of isopropyl- 3-D-thiogalactopyranoside (IPTG) (USB) at set cell densities (OD660between0.01 and 0.8) corresponding to cultures at 103-107 CFU/mL. 

Concentrated PBS (10X), 0.5 Af ethylendiamine tetraacetic acid (EDTA), 1 Af Tris-HCl pH 8.0, 5 Af NaCl, 3 AfNaAc pH 5.2, kanamycin and 1 M isopropyl-(3-D-thiogalactopyranoside (IPTG) from Gibco/BRL (Bethesda, MD). 

GST-C3 fusion protein expression is induced by adding isopropyl- 3-D-thiogalactopyranoside (IPTG Calbiochem) to 0.2 mM (1 ml of 0.1 M stock made in water and stored at -20 "C), and the culture is incubated with shaking for a further 3h. 

Biological. Dibenzofuran was oxidized by salicylate-induced cells of Pseudomonas sp. strain 9816/11 and isopropyl-P-D-thiogalactopyranoside-induced cells of Escherichia co//JM109 (DE3) (pDTG141) to (li ,25)-ds-l,2-dihydroxy-l,2-dihydrodibenzofuran (60-70% yield) and 3SARj-ds-3,4-dihydroxy-3,4-dihydrodibenzofuran (30-40% yield) (Resnick and Gibson, 1996). 

Fig. 7.5 (a) The vector is silent in uninfected cells because the transactivator ctat gene is repressed. Addition of the inducer (isopropyl-j8-D-thiogalactopyranoside [IPTC] or tetracycline) abolishes the repression of ctat (1) and Tat protein is produced (2). The Tat protein produces the transcription driven by the 5 -LTR of the vector, which includes the p53 gene (3). P53 protein is not synthesized because the intron including p53 is spliced (4). No apoptosis is produced, and when the inducer is removed there is no longer any production of Tat. (b) If a latent provirus is in the cell, the addition of the inducer (1) allows production of the vector Tat (2). The Tat protein produces the transcription driven by the 5 -LTRs of the vector and provirus (3), and Rev protein accumulates... 

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