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Ethylendiamine tetraacetic acid

Concentrated PBS (10X), 0.5 Af ethylendiamine tetraacetic acid (EDTA), 1 Af Tris-HCl pH 8.0, 5 Af NaCl, 3 AfNaAc pH 5.2, kanamycin and 1 M isopropyl-(3-D-thiogalactopyranoside (IPTG) from Gibco/BRL (Bethesda, MD). [Pg.285]

Figure 16 shows the long-term stability of the FET using a 1.7 mM urea solution. The response amplitude of the sensor decreases rapidly after 40 measurements without adding EDTA (ethylendiamine tetraacetic acid) to buffer solutions (Fig. 16, —EDTA). Adding EDTA to the buffer increases the life of the urea sensor. The urea sensor is usable for more than 2000 assays, showing no decrease in the response amplitude (Fig. 16, +EDTA). The rapid decrease in the response amplitude in the absence of EDTA suggests that heavy metal ions inactivate urease. The effect of EDTA on the durability of a urea sensor has been previously reported (48). Figure 16 shows the long-term stability of the FET using a 1.7 mM urea solution. The response amplitude of the sensor decreases rapidly after 40 measurements without adding EDTA (ethylendiamine tetraacetic acid) to buffer solutions (Fig. 16, —EDTA). Adding EDTA to the buffer increases the life of the urea sensor. The urea sensor is usable for more than 2000 assays, showing no decrease in the response amplitude (Fig. 16, +EDTA). The rapid decrease in the response amplitude in the absence of EDTA suggests that heavy metal ions inactivate urease. The effect of EDTA on the durability of a urea sensor has been previously reported (48).

See other pages where Ethylendiamine tetraacetic acid is mentioned: [Pg.259]    [Pg.160]    [Pg.3]    [Pg.259]    [Pg.160]    [Pg.3]    [Pg.613]   
See also in sourсe #XX -- [ Pg.849 , Pg.855 , Pg.897 , Pg.1114 ]




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