Isolation and Purification of Lactic Acids

The fermenting liquor finally obtained in the above fermentation contains lactic acid together with various impurities such as un-reacted raw materials, cells and culture media-derived saccharides, amino acids, carbojq lic acids, proteins and inorganic salts. Therefore, the isolation and purification steps are needed for obtaining a highly pure product needed in the polymer s synthesis. In the usual fermentation process, the generated lactic acid is neutralized in situ with calcium oxide or ammonia. When calcium oxide is used for the neutralization, calcium lactate is precipitated out. 

Strain Glucose Fructose Maltose Sucrose Cellobiose 

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