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Isoelectric points modifier effect

Physical and ionic adsorption may be either monolayer or multilayer (12). Capillary stmctures in which the diameters of the capillaries are small, ie, one to two molecular diameters, exhibit a marked hysteresis effect on desorption. Sorbed surfactant solutes do not necessarily cover ah. of a sohd iaterface and their presence does not preclude adsorption of solvent molecules. The strength of surfactant sorption generally foUows the order cationic > anionic > nonionic. Surfaces to which this rule apphes include metals, glass, plastics, textiles (13), paper, and many minerals. The pH is an important modifying factor in the adsorption of all ionic surfactants but especially for amphoteric surfactants which are least soluble at their isoelectric point. The speed and degree of adsorption are increased by the presence of dissolved inorganic salts in surfactant solutions (14). [Pg.236]

Effect of pH The pH of a solution affects the solubilization characteristics of a protein primarily in the way in which it modifies the charge distribution over the protein surface. At pH values below its isoelectric point (pi), or point of zero net charge, a protein acquires a net positive charge, while above its pi the protein will be negatively charged. Thus, if eleetro-static interactions are the dominant factor, solubilization should be possible only with anionic surfactants at pH values less than the pi of the protein because at values above pi, eleetrostatic repulsion would inhibit solubilization. The opposite effeet would be anticipated in the case of cationie surfactants. [Pg.664]

Emulsifying activity index (EAI) is a measure of the ability of protein to emulsify oil, which depends on solubility, size, charge, and surface activity of the protein molecules. The effect of proteolysis with pronase E on EAI of the modified protein was relatively insignificant (Figure 6) However, deamidation appeared to enhance EAI, especially at pH values more basic than the isoelectric point (pH 4.7). [Pg.186]

Fig. 6. Effect of modifying temperature on EDA with the following modifying reagent and conditions ( ) (+)-erythro-2-methyltartaric acid, pH 5.0 5.2, 0°C (O) (S.S)-tartaric acid, pH 5.0-5.2, 0°C (A) ( + )-2-methyl glutamic acid, pH 5.0, 0°C (O) (S)-valine, isoelectric point, 0 C ( ) (S)-glutamic acid, pH 5.2, 0 C. Reaction conditions MAA (neat), 60"C, 80 100 kg/cm2. Fig. 6. Effect of modifying temperature on EDA with the following modifying reagent and conditions ( ) (+)-erythro-2-methyltartaric acid, pH 5.0 5.2, 0°C (O) (S.S)-tartaric acid, pH 5.0-5.2, 0°C (A) ( + )-2-methyl glutamic acid, pH 5.0, 0°C (O) (S)-valine, isoelectric point, 0 C ( ) (S)-glutamic acid, pH 5.2, 0 C. Reaction conditions MAA (neat), 60"C, 80 100 kg/cm2.
Proteolytic modification has special importance for the improvement of solubility of proteins. This effect becomes significant even after very limited proteolysis. Hydrolysis of casein to DH of 2 and 6.7% with Staphylococcus aureus V8 protease increased the isoelectric solubility to 25 and 50%, respectively (Chobert et al., 1988a). However, it should be noted that the solubility profiles were not identical, due to a shift of the isoelectric point of the modified proteins. Solubility of a protein hydrolysate depends on the enzyme used (Adler-Nissen, 1986a). Protamex (a Bacillus proteinase complex) hydrolysates of sodium caseinate (DH 9 and 15%) displayed 85-90% solubility between pH 4 and 5 (Slattery and FitzGerald, 1998). [Pg.38]

Specific interactions between starch and proteins were observed as early as the beginning of the twentieth century. Berczeller996 noted that the surface tension of aqueous soap solutions did not decrease with the addition of protein (egg albumin) alone, but it did decrease when starch and protein were added. This effect was observed to increase with time. Sorption of albumin on starch is inhibited by bi- and trivalent ions and at the isoelectric point. Below the isoelectric point, bonding between starch and albumin is ionic in character, whereas nonionic interactions are expected above the isoelectric point.997 The Terayama hypothesis998 predicts the formation of protein complexes with starch, provided that starch exhibits the properties of a polyelectrolyte. Apart from chemically modified anionic starches (such as starch sulfate, starch phosphate, and various cross-linked starch derivatives bearing ionized functions), potato starch is the only variety that behaves as a polyelectrolyte. Its random phosphate ester moieties permit proteins to form complexes with it. Takeuchi et a/.999-1002 demonstrated such a possibility with various proteins and a 4% gel of potato starch. [Pg.408]

Class 2 modifiers are simple inorganic acids and bases that can alter the chemistry of the support surface. The presence of acid significantly affects the quantity of chloroplatinic acid adsorbed on alumina with the effect of solution pH on the extent of adsorption (Fig. 13.10). As discussed in Chapter 9 and illustrated fiirther in the discussion on ion exchange presented later, the isoelectric point (lEP) for alumina occurs at a pH near 8 and no adsorption occurs at higher pH values. The amount of nickel nitrate or nickel chloride adsorbed on 7 alumina is very low below pH 4, with the amount adsorbed increasing abruptly at a pH near 5.2. 2> 3... [Pg.284]


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