Isobutanol Production with Corynebacterium glutamicum

TABLE 12A Relevant Characteristics of Selected Isobutanol-Producing Strains of E. coli, C. glutamicum, B. subtilis,R. euthropha, S. cerevisiae, S. elongates, and C. ceUulolyticum 

Strain Process Substrate Yp/5 (mole/mole), (g/g) Titer (g/L) Reference 

coli JCL260/pSA55/pSA69 Flask Glc 0.86, 0.35 22 Atsumi et al. (2008a) 

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Blombach B, Eikmanns BJ. (2011). Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum. Bioeng Bugs, 2, 346-350. 

With the successful development of an isobutanol production pathway in E. coli, the a-keto-acid isobutanol production pathway was transferred to the traditional amino acid producer Corynebacterium glutamicum [89]. The native ilvCD and adhA genes were overexpressed, which led to 2.6 g 1 of isobutanol production with other alcohols as byproducts. By deleting the competing pathways, namely pyruvate carboxylase and lactate dehydrogenase, isobutanol production was increased to 4.9 g 1 . The work showed the potential of both the universality of the a-keto-acid isobutanol production pathway and the potential of utilization of C. glutamicum for higher chain alcohol production [89]. 

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