Isobutanol Production with Escherichia coli

Applying anaerobic conditions, the maximal theoretical yield can be calculated to be 0.41 g isobutanol per gram of glucose (1 mole/mole Li et al., 2010). 

Overview of the Biochemical Properties of Relevant Enzymes with 2-Ketoacid Decarboxylase or Alcohol Dehydrogenase 

alcohol dehydrogenase AlsS, acetohydroxyacid synthase KDC, 2-ketoacid decarboxylase YqhD, broad-range alcohol dehydrogenase TPP, thiamine pyrophosphate. 

TABLE 12.3 Relevant Yields and Titers of Isobutanol, 2-Methyl-l-Butanol, 3-Methyl-l-Butanol, 1-Butanol Derived of the Metabolic Pathways of Branched-Chain Amino Acids 

Strain Process Product (mole/mole), (g/g) Titer (g/L) Reference 

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Blombach B, Eikmanns BJ. (2011). Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum. Bioeng Bugs, 2, 346-350. 

Baez, A., Cho, K.-M., and Liao, J.C. (2011) High-flux isobutanol production using engineered Escherichia coli a bioreactor study with in situ product removal. Appl Microbiol Biotechnol, 90, 1681-1690. 

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