Iron ammonium alum acetate

Aminoethanol 2-Aminoisobutyric Acid Ammonia Ammonia Solution Ammonium Ammonium Acetate Ammonium Alum Ammonium Bicarbonate Ammonium Bichromate Ammonium Bifluoride Ammonium Bromide Ammonium Carbonate Ammonium Chloride Ammonium Dichromate Ammonium Fluoride Ammonium Glycolate Ammonium Hydrogen Carbonate Ammonium Hydrogen Fluoride Ammonium Hydrosulfide Ammonium Hydroxide Ammonium Hyposulfite Ammonium Iron Sulfate Ammonium Molybdate Ammonium m-Phosphate Ammonium Nitrate Ammonium o-Phosphate Ammonium Oxalate Ammonium Peroxydisulfate Ammonium Persulfate Ammonium Phosphate Ammonium Phosphate Dibasic Ammonium Rhodanide Ammonium Sulfamate Ammonium Sulfate Ammonium Sulfide Ammonium Thiocyanate Ammonium Thioglycolate Ammonium Thiosulfate... 

A more refined test is the Schultz (1924) method for cholesterol, which involves the application of a mixture of concentrated sulfuric acid and glacial acetic acid to sections which have been oxidized with ferric ammonium sulfate (iron alum). A blue-green color results. The iron alum apparently oxidizes 3-hydroxy steroids and their esters to 7-oxy steroids, which give the Lifschlltz color reaction on the application of the acids. (Fieser and Fieser, 1949, p. 234). This test is considered to be more specific for a limited group of steroids than is the sulfuric acid method cited above, which reveals a large number of unsaturated polycyclic compounds. Recently, however, Kent (1952) has reported that a positive reaction also occurs with carotene. 

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