Ionization matrix-assisted laser adsorption

MALDI-TOF Matrix-assisted laser adsorption/ionization time-of-flight... 

This proposal describes the development of a new, systematic approach for qualitatively and quantitatively studying surface-biomolecule interactions by matrix-assisted laser desorption ionization (MALDl) mass spectrometry (MS). This methodology is being developed because of the profound importance that surface-biomolecule interactions play in applications where biomaterials come into contact with complex biological fluids, it can readily be shown that undesired reactions occurring in response to surface-biomolecule contact (protein adsorption, biofouling, immune response activation, etc.) lead to enormous economic and human costs. Thus, the development of analytical methodologies that allow for efficient assessment of the properties of new biomaterials and/or the study of detailed fundamental processes initiated upon surface-biomolecule contact are of critical value ... 

The polypeptide polyalanine was synthesized1 and characterized by Matrix Assisted Laser Desorption Ionization (MALDI) and amino acid hydrolyses. Monolayers of either l- or D-polyalanine polypeptides, in the form of an a-helix, were prepared by self-assembly on a borosilicate slide that was coated with a 200-300 nm thick gold film (Metallhandel Schroer Gmbh). Prior to the adsorption, the gold slides were annealed by flame and only then inserted into a 1 mM peptide solution of... 

Matrix-assisted laser desertion ionization (MALDI) was first described by Karas and Hillenkamp in 1988 [469]. At that time it was a revolutionary method for the ionisation and analysis of large biomolecules. Now many more MS methods have been devised, but the analysis of complete protein masses is still only possible by MALDI [470]. With MALDI, a matrix (crystals of small organic molecules) with a small amount of analyte is ionised by a short laser pulse at a wavelength close to the adsorption band of the matrix molecules. This produces predominantly singly charged molecular ions, which are detected by the TOE spectrometer. The ana-... 

Spriestersbach KH, Rode K, Pasch H. Matrix-assisted laser desorption/ionization mass spectrometry of synthetic polymers, 7. Analysis of fatty alcohol ethoxylates by coupled liquid chromatography at the critical point of adsorption and MALDI-TOF mass spectrometry. Macromol Symp 2003 193 129-41. 

Macromolecular Materials and Engineering 286, No.3, 30th March 2001, p.161-7 MATRIX-ASSISTED LASER DESORPTION/ IONIZATION MASS SPECTROMETRY OF SYNTHETIC POLYMERS, 5A. ANALYSIS OF POLY(PROPYLENE OXIDE)S BY COUPLED LIQUID CHROMATOGRAPHY AT THE CRITICAL POINT OF ADSORPTION AND MALDI-TOF MASS SPECTROMETRY Keil C Esser E Pasch H Darmstadt,Deutsches Kunststoff-Institut... 

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