Introduction to Genomics Technologies

The transport activity of drug carriers can be affected in many different ways. Thus, with respect to drug design, different questions have to be addressed Which transporters can be utilized in the intestine for oral drug absorption Can the expression of these transporters be affected by either disease states or type of nutrition, age, and sex Is it possible that in a population, various genotypes exist with different transport activities and substrate specificities  

It is important to note that RNA may not always correlate with the expression of encoded proteins. Use of proteomic techniques increasingly serves to resolve discrepancies between mRNA levels and expressed proteins. However, proteomics of integral membrane proteins still remains a challenge moreover, comparing mRNA levels in similar tissues from different subjects is likely to provide an indication of protein levels. Any polymorphism that affects protein structure and function could also lead to false interpretations if the array fails to measure the functional polymorphism. 

Gene Expression Profiling Along the Intestine and in Caco-2 Cells 

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