Intrinsic cytotoxicity assessments

CRITICAL ASSESSMENT OF THE METHOD Care should be taken to avoid conditions (pH, high cytotoxicity, osmolality etc) that would lead to positive results but which do not reflect intrinsic mutagenicity. Mammalian carcinogens are often positive in this test. Nevertheless, there is not a perfect correlation between this test and carcinogenicity, which depends on the chemical class. Some chemicals may test positive in this test because they appear to act through other mechanism than direct DNA-damage, for example apoptosis. 

In 2004, Stolte S. et flZ.[155] build a different toxicity model using cytotoxicity data gather from studies including several anions and l-alkyl-3-methylimidazolium cations (with ethyl, butyl and hexyl chains) and perfomed in the IPC-81 rat leukemia cell line. Firstly, intrinsic toxicity of the anions and cations were assessed assuming that sodium chloride and lithium chloride and, the chloride as anion, respectively, had no imp>act on toxicity behaviour. These studies revealed that only 10 anions from a total of 27 demonstrated considerable toxicity... 

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