Internode extension

Internode Extension in Pisum sativum Correlates with GA20 3/8-hydroxylation [23]... 

An aqueous extract of P. hysterophorus (collected in Puerto Rico) was partitioned into methylene chloride at pH 7, pH 10 and pH 2. Bioassays of the methylene chloride soluble fractions, using the bean second internode bioassay (13), showed that the highest activity was concentrated in the methylene chloride extract at pH 7. Extensive chromatographic purification (flash chromatography, medium pressure LC, preparative TLC) monitored by bioassay led to the isolation of the four sesquiter-... 

Myelinating oligodendrocytes have been studied extensively [5,30] (see Ch. 4). Examination of the CNS during myelinogenesis (Fig. 1-15) reveals connections between the cell body and the myelin sheath [31] however, connections between these elements have never been demonstrated in a normal adult animal, unlike the PNS counterpart, the Schwann cell. In contrast to the Schwann cell (see below), the oligodendrocyte is capable of producing many internodes of myelin simultaneously. It has been estimated that oligodendrocytes in the optic nerve produce... 

Bioassay Studies of Brassins - BARC As indicated by Mandava (3), bioassay systems have been rather extensively used to study brassins physiology. In addition to the bean 2nd internode bioassay, the bean first internode curvature bioassay (28) and the mung bean epicotyl bioassay (29) were found to be useful by Meudt and Gregory. In many cases, details of early brassins studies using these and other assay systems were not published because the chemical nature of the active component(s) of brassins was unknown at the time. However, types of responses obtained with the synthetic brassinosteroids were essentially the same as those obtained with brassins (29, 30, 31, 32, 33). ... 

There is extensive evidence that GA increases auxin levels either by enhancement of auxin biosynthesis (Jindal and Hemberg 1976, Sastry and Muir 1965) or by retardation of auxin destruction (Kogl and Elema 1960). Nevertheless, there is convincing evidence that GA can also stimulate elongation independently of auxin (Kazama and Katsumi 1974, Cleland et al. 1968, Kaufman et al. 1969). In excised Avena stem internodes, for example, GA stimulates cell elongation, while auxin is inhibitory (Kaufman et al. 1969). Similarly, stimulation of lettuce hypocotyl elongation has been used as a bioassay for GA, while... 

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