Interleukin-3 concentration curves

Sample loading pipette 40 pL of Interleukin IB sample solution in each of the eight reservoirs. In order to establish a calibration curve, interleukin concentrations ranging from 0.2 to 17.5 pM are used in the present example (IL-1B1,1 standards 1-8). The various solutions are then pumped through the micro-channels using 60... 

Fig. 11.15. A multiplex bead array that can be used to capture multiple soluble analytes. In the dot plot, 15 types of beads are distinguished by their red/orange ratios they capture 15 different cytokines. For example, the beads in Region 1 capture interleukin (IL)-9 in Region 5, IL-2 in Region 9, IL-5 and in Region 13, MCP-1. The green fluorescence histograms from each region show, by their intensity, how much cytokine has been captured by each type of bead. Standard curves can relate the green fluorescence intensity to the concentration of cytokine in the sample. The experimental format illustrated in the cartoon here is patterned directly from work by RT Carson and DAA Vignali (1999).

The pharmacokinetics and pharmacodynamics of recombinant interleukin-2 (IL-2) in patients with human immunodeficiency virus (HIV) infection have been evaluated (75). Patients were administered IL-2 either by continuous infusion or by SC injection for 5 days over multiple cycles. Following repeated injection, soluble IL-2 receptors were substantially but transiently increased. A dose-dependent decrease in area under the concentration-time curve (AUC) between days 1 and 5 was attributed to a receptor-mediated change in clearance. Concentrations were described using an unusual model that employed an indirect stimulatory PD model to link the time-dependent changes of the pharmacokinetics with the change in IL-2 receptor density following repeated administration. 

Fig. 5 Interleukin 1-beta concentration assay. A Binding of human IL-l/S to mouse anti-IL-l/S antibody, previously captured onto a RaM-Fc surface. Binding curves for five different IL-1/3 concentrations are shown overlaid, synchronised to the start of each IL-l/S injection. Data have been normalised by subtraction of the response to injection of buffer alone, to correct for the off-rate of the captured anti-IL-l/S antibody. B IL-l/S concentration-response calibration curve. The initial binding rate for each IL-l/S concentration was calculated as for the myoglobin concentration-response calibration curve (Fig. 4)...

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