Interface confocal microscopy

Schroth D 1997 The confocal laser scanning microscopy. A new tool in materials testing Matehalpruefung 39 264 Chestnut M H 1997 Confocal microscopy of colloids Curr. Opin. Colloid Interface Sc/. 2 158-61... 

Confocal microscopy (CM) is another microscope technique for apparent optical sectioning, achieved by exclusion of out-of-focus emitted light with a set of image plane pinholes. CM has the clear advantage in versatility its method of optical sectioning works at any plane of the sample, not just at an interface between substances having dissimilar refractive indices. However, other differences exist which, in some special applications, can favor the use of TIRF ... 

The structure of CdSe nanoparticles segregated to the fluid interface as shown by confocal microscopy (Fig. 4a) has been investigated ex situ with scanning force microscopy (SFM) and transmission electron microscopy (TEM) methods. All results point to a monolayer of nanoparticles with liquidlike ordering at the interface (Fig. 4b,c) [46],... 

In imaging the 3-dimensional organization of the actin system by confocal microscopy, the location of a structure relative to the plasma membrane may be of interest. Therefore, if images are reconstructed in z-direction from stacks of confocal sections, a reference to exactly locate the cell-to-substrate interface is advisable. 

The numerical simulation of the chronoamperometirc tip current revealed that the high rate of Ag reduction (5-10 cm/s) at the ITIES weakly depends on the Galvani potential dilference across the ITIES. Moreover, the local deposition of Ag particles at the interface under the SECM tip was visualized by confocal microscopy (Fig. 16b). The deposition of much smaller Ag particles will be possible using a newly developed Ag nanoelectrode as an SECM tip. ... 

Campbell, A. I. Bartlett, P. Fluorescent hard-sphere polymer colloids for confocal microscopy. J. Colloid Interface Sci. 2002, 256, 325-330. 

Figure 3.8 As-synthesized water wax Si02 core-shell particles obsenred by fluorescence confocal microscopy at different magnifications. The white arrow underlines the double-core internal water-in-wax interface.

Fluorescent nanoparticles, such as CdSe QDs, have been used to probe fluid-fluid interfadal nanopartide segregation, as well as the relationship between nanopartide size and various droplet characteristics, including nanopartide transport across the interface, in-plane interfadal mobility, and phase separation of different-sized nanoparticles. Fluorescence confocal microscopy is particularly useful for observing the stmrture and behavior of QD-stabilized... 

The stability of the bijel stracture over time was probed by periodic characterization of the FrrC-Si02/lutidine/water stme-tures. With storage in an incubator at 40 ° C, the structures were maintained, as observed by confocal microscopy, over at least 7 months. These studies revealed the bijel domain morphology to be long-lived, but could not mle out completely the possibility of a very slow aging process and nanopartide reorganization within the lutidine-water interface. ... 

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