Inter- dye distance

Thus, true FRET efficiencies or inter-dye distances (R) are generally not calculated, although with careful analysis, control experiments and an awareness of the uncertainties, R can be computed (e.g. see [79, 80]. A discussion of these complexities can be found in Section 2.5.7). 

The closed conformation of syntaxin when complexed with munc-18 was characterized by measurement of the lifetime of the donor with the acceptor present TpA and the inter-dye distance (see Figure 5.8(c)). The tight clustering of... 

To demonstrate that STORM can indeed resolve nearby fluorescent molecules with sub-diffraction-limit resolution, we first engineered samples with known relative positions of the fluorescent labels - double-stranded DNA labeled with two Cy3-Cy5 pairs separated by a well-defined number (135) of base pairs, corresponding to an inter-CyS distance of 46 nm along the contour of DNA [4]. The DNA strands were immobilized in a flat configuration to a quartz slide through multiple biotin-streptavidin linkages. The two Cy5 dyes were turned on and off, repetitively, and the image sequence was analyzed to determine the positions of individual activated Cy5 dye. We then constructed... 

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