Integrins three-dimensional structure

The RGD motif has long been known to be the principal binding motif of several integrins (77). Aumailly et al. showed that the cyclic peptide c(RGDfV), where f denotes d-phenylalanine (D-Phe), is a potent inhibitor of the integrin aubfo and determined the three-dimensional structure of the cyclic peptide from... 

THE THREE-DIMENSIONAL STRUCTURE OF INTEGRINS AND THEIR LIGANDS, AND CONFORMATIONAL REGULATION OF CELL ADHESION... 

Donahue, J. P., Patel, H., Anderson, W. F., and Hawiger, J. (1994). Three-dimensional structure of the platelet integrin recognition segment of the fibrinogen 7 chain obtained by carrier protein-driven crystallization. Proc. Natl. Acad. Sci. USA 91, 12178-12182. 

I The Three-Dimensional Structure of Integrins and their Ligands, and Conformational Regulation of... 

N. (1996). Detailed oligosaccharide structures of human integrin aSfil analyzed by a three-dimensional mapping technique. Eur. J. Biochem., 237, 76-85. 

Although found in many nonepithelial cells, integrin-containing adhesive structures have been studied most frequently in fibroblasts grown in cell culture on flat glass or plastic surfaces (substrata). These conditions only poorly approximate the three-dimensional ECM environment that normally surrounds such cells in vivo. When fibroblasts are cultured in three-dimensional ECM matrices derived from cells or tissues, they form adhesions to the three-dimensional ECM substratum, called 3D adhesions. These structures dif-... 

Fig. 2. Integrin architecture. (A) Organization of domains within the primary structure. Some a subunits contain an I domain inserted in the position denoted by the dotted lines. Cysteines and disulfide bonds are shown as lines below the stick figures. Red and blue asterisks denote Ca and Mg " " binding sites, respectively. (B) Arrangement of domains within the three-dimensional crystal structure of (Xiong et al., 2001). Each domain is color coded as in A. (C) The structure in (B) with an I domain added.

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