Integration peak detection

Detection RI 16X, SP4050 Attenuation 10 Chart speed - Icm/min Analysis time - 24 min Total plate count - 2(10 ) plates Polydichlorophosphazene and cyclic trimer and tetran r standards were used for calibration. Standard methods were applied for integrating peak areas. 

Despite these advancements in chromatographic data processing, peak detection and integration algorithms were crude, the user interface was cumbersome, and there was very little flexibility in the types of reports that these systems could generate. 

Optional availability of free cysteines can be assayed by reacting with an SH-directed /V-(l-pyrene)-maleimide. Add N-( l-pyrcnc)-maleimide to deprotection reaction mixture to make a molar protein-to-pyrene ratio of 1 2 and incubate for 40 min at room temperature. Load on RP HPLC C4 Alltech MACROSPHERE 300 5-mm column and elute at 0.75 mL/min with 0.1% TEA (v/v) and a linear gradient of acetonitrile (5-50% over 15 min) with detection at 216 nm for protein and 340 nm for pyrene. Calculate the extent of pyrene modification using a ratio of integral peak intensities at 216 nm and 340 nm. 

A bubble in the detector s flow cell typically manifests itself as a spike which may hinder peak detection and integration. Dissolved oxygen poses special problems such as baseline shifts caused by the formation of ultraviolet (UV) absorbing 02-solvent complexes and diminished fluorescence response (quenching). 

The ability to do on-capiUary detection and to calculate integrated peak areas from the collected data makes CGE-LIF very useful for the quantification of PCR- and RT-PCR-generated dsDNA products. The quantity of the amplified product can be indicative of the efficiency of PCR and this information can be used to optimize the reaction. Such information is also useful in determining the amount of a specific DNA or RNA present in the analyzed sample. Quantitation can be achieved by relative or absolute estimates. A ratio of target DNA peak area to the peak area of an added dsDNA internal standard gives an estimate of the relative amount of target DNA generated by PCR. 

Most integrators are developed for use in GC and have sophisticated routines for peak detection and for baseline corrections if there are negative slopes. They are able to detect small peaks even in the descending part of a solvent peak. How-... 

Electronic integration zero 0.1 very good (depending on the algorithm for curve smoothing, peak detection, and baseline drift) suited... 

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