Insert scan

Mingarro I, Whitley P, Lemmon MA, Von Heijne G. 1996. Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix a rapid way to map helix—helix interactions. Protein Science 5 1339. 

Membrane Insertion Scanning. Membrane topology of transporter proteins can be... 

The insert scan command replaces the non-scan sequential cells with scan equivalent cells and connects the scan cells to form a scan chain. 

Save the design database in db format prior to inserting scan, write -f db -hier current.design -output top.db... 

Perform Scan Insertion using the insert.scan command, insert scan... 

You execute insert scan at the core level of your design followed by check test. TC report no errors. Then you create another hierarchy around your core design and manually instantiate pads. On executing check test at the top level of the design with the pads included in the netlist, TC reports, No scan-path found . 

Figure 5.141. A glass fiber reinforced LCP composite is shown to have interesting morphology. A polished thin section is shown in polarized light (A) to exhibit a fine domain texture with some orientation of the polymer on the glass surfaces (see color insert). Scanning electron microscopy fracture views (B-D) show the tenacious adhesion of the LCP to the fibers. Fibrillar structures are oriented parallel to the fiber surface, and submicrometer sized domains are observed (D).

A generator is used for all reporting. Since set up and result information is stored in a Resource File by name, the report generator can scan this file and insert the values wherever the name appears in the user s template... 

Figure 26. Constant current mode STM image of isolated (A), self-organized in close-packed hexagonal network (C) and in fee structure (E) of silver nanoclusters deposited on Au(l 11) substrate (scan size (A) 17.1 x 17.1 nm, f/t=—IV, /t=ltiA, (C) 136 X 136 nm, f/t = — 2.5 V, /t = 0.8 tiA, (E) 143 x 143 nm, = —2.2 V, /, = 0.72 nA). I U) curves and their derivatives in the inserts of isolated (B), self-organized in close-packed hexagonal network (D) and in fee structure (F) of silver nanoclusters deposited on Au(l 11) substrate. (Reprinted with permission from Ref. [58], 2000, Wiley-VCH.)...

Figure 6.10 Galvanostatic scans of a Pt(l 10) rotating disk electrode in a CO-satuiated 0.1 M HCIO4 solution at two different current scan rates (disk rotation rate 400rev/min). The insert shows the potential fluctuations observed at an apphed current density of 0.74 mA/cm (disk rotation rate 900 rev/min).

Figure 14.9 CO bulk electro-oxidation at bare Ru(OOOl) in flow cell dotted line, CO fi ee electrolyte solid lines flow of CO saturated electrolyte, with varied upper scan limits (see key on flgure). (See color insert.)...

Figure 16.8 Pt/TiO c-catalyzed oxygen reduction potential, where 0.01 mA cm is reached during the negative scan in a cyclic voltammetry experiment (scan rate 20 mV s ) in oxygen-saturated 0.5 M HCIO4 at 25 °C. (See color insert.)...

FIG. 5 Potential difference between two Ag/AgCl electrodes in the stem of a soybean plant after insertion of the electrodes. Distance between electrodes was 8 cm. Volume of soil was 0.5 L. The plants were given water every other day and kept at 24°C. Frequency of scanning was 1000 samples per second. 

Wide use of TLC-MS is hampered by the lack of commercially available interfaces. This also restricts automation and high throughput. Commercial direct insertion probes for scanning TLC-MS are available [811]. Compared with on-line LC-MS operation, TLC-MS hyphenation is much less highly developed. 

FIG. 3. Chromosome arms begin to separate in pro metaphase. Scanning electron micrographs of human chromosomes isolated from cells in prophase (A), prometaphase (B), metaphase (C) and early anaphase (insert in C). Size bar, 1 /tm. Reprinted with permission from Sumner (1991). 

Fig. 2.10. Potential step SNLFTIRS spectra from a polished polycrystalline Pt electrode, immersed in 10 2 M CHjOH/O.l M HC104 electrolyte. All spectra (90 scans each, 8 cm 1 resolution) were normalized to the base spectrum collected at 0 mV vs. RHE. Insert part of the curve at 450 mV in expanded scale.

The SEEPR technique allows the simultaneous recording of the CV and the CW EPR spectrum of the radicals produced during the electron transfer reactions (Khaled et al. 1991). The SEEPR technique consists of an IBM enhanced electrolytic cell inserted in a rotating cylindrical EPR cavity. The cell is no longer sold by IBM, but a description can be found (Khaled et al. 1990, 1991). The CVs were obtained using a commercial (BAS-100) electrochemical analyzer while simultaneously recording the EPR spectra during the scan. 

Hallet, B., Sherratt, D.J. and Hayes, F. (1997) Pentapeptide scanning mutagenesis random insertion of a variable five amino acid cassette in a target protein. Nucleic Acids Research, 25, 1866-1867. 

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