Inositol normal range

The normal range of inositol in plasma by this technique was 0.42-0.76 pg/ml. Higher values up to 1.9 (ig/ml were found in individual patients. Destruction of some inositol during acid hydrolysis can not be excluded. The state of inositol in the plasma and the possible clinical significance of inositol need further investigation. 

In addition, most severe white matter abnormalities were observed in patients with small values for K, and high ratios of T IV The brain concentrations of the classical metabolites measured by proton spectroscopy, namely A-acetylaspartate, inositol, lactate, and creatinine, were found to be normal. Even the concentration of choline, described to be elevated in acute demyehnating disorders with enhanced membrane lipid turnover, was in the normal range. 

Loweth et al. [120] showed that fluoride induces apoptosis in clonal pancreatic fi cells and in the cells of normal rat islets of Langerhans. The process may reflect the formation of AlF41 since it was inhibited by the aluminum chelator desferrioxamine. Recent studies provide evidence that apoptosis of pancreatic fi cells is important in the early etiology of diabetes mellitus. Treating thymus lobe cells with aluminofluoride complexes also provoked apoptosis of a wider range of thymocyte subtypes [121] with an accumulation of inositol phosphates. The responses to aluminofluoride complexes were not prevented by inhibitors of tyrosine kinases, suggesting that unidentified G-proteins which couple to phospholipase C activation may also be capable of initiating apoptosis by a route independent of the T cell receptor. 

Figure 20.9. Two-dimensional mass spectrometric analysis of GPIns molecular species in a lipid extract of mouse myocardium. The lipid extract of mouse myocardium was prepared by a modified Bligh and Dyer procedure as previously described. Each MS or MS/MS trace of the 2D ESI mass spectrum was acquired by sequentially programmed, customized scans operating under Xcalibur software. For negative-ion tandem mass spectrometry in the precursor-ion (PI) mode, the first quadrupole was scanned in the selected mass range and the second quadrupole was used as a coUision cell while the third quadrupole was fixed to monitor the ion of interest (i.e., either inositol phosphate, glycerophosphate, or a fatty acyl carboxylate fragmented from GPIns molecular species). All mass spectral traces were displayed after being normalized to the base peak in each individual trace.

---