Inoculating needle

The slant was inoculated with ATCC 23342 and incubated for 6 days at 30°C. The culture does not normally sporulate on this medium, and it is necessary to macerate the mycelial mat with a flattened, sharpened, inoculating needle in order to increase the number of potential growth centers. The macerated mature culture was covered with sterile distilled water and scraped carefully with a sterile rod to obtain a mycelial suspension. 

Stock culture of B. subtilis strain 168 (Bacillus Genetic Stock Center strain 1A1, Columbus, OH). Inoculating needle—1 required. 

Inoculate the liquid with a single bacterial colony by touching the colony with a sterile inoculating needle, making certain that some of the cells have been transferred to the needle. Take at least two different colonies and grow them each in a different tube. 

Scrape the frozen surface of the glycerol culture with a sterile inoculating needle and streak the bacteria across one side of an LB agar plate containing ampicillin. 

Autoclaved sterile toothpicks or inoculating needles (cat 08-757-135, Fisher Scientific, Pittsburgh, PA). 

Insert an inoculating needle or autoclaved toothpick into a stab of E. coli. 

Streak a NZY agar plate containing 1 OOpg/mL kanamycin with the toothpick or inoculating needle, which is now coated with E. coli (Fig. 1). 

After inserting an inoculating needle or toothpick in the E. coli stab, streak the coated needle (or toothpick) across a NZY agar plate multiple times in one direction. 

Using the same inoculating needle or toothpick, streak across the original stripes on the NZY agar plate multiple times in a perpendicular direction. 

Sterile 1.5 mL tubes, inoculating needles, spreaders, disposable petri dishes. 

Balance Beakers Bunsen burner Inoculating loop Inoculating needle Measuring cylinders pH meter... 

Using an inoculating needle, gently stab an individual colony from transformation plates taking care not to take too much colony. Twist the needle about three times in the PCR mix in the appropriate well of the microplate. 

Transfer a small amount of the microorganism with an inoculating needle. Mix with the water on the slide and spread over a large area. 

Some lactic acid bacteria have the ability to metabolize sucrose to produce polymers known as dextrans (viscous, slimy). While most strains of Oeno-coccus oeni are negative for this attribute (Edwards et al., 1991), some strains of Lactobacillus (Edwards et al., 1993) and Leuconostoc mesenteroides (Garvie, 1984) are positive. The outlined method originates from Pilone and Kunkee (1972). To test for dextran formation, touch a sterile inoculating needle straight down into an isolated colony and move the needle straight back up. A positive reaction is one where threads or strands are drawn up on the needle. 

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