B. subtilis strain

From a culture filtrate of B. subtilis strain F-ll, Kaji and Saheki4 purified endo-L-arabinanase to a homogeneous protein by hydroxylapatite... 

All carbon sources tested have favored extracellular production of active surface agent by B. subtilis ATCC 6633, which was estimated by the reduction in surface tension of the fermented broth. However, except for stillage, there was no relationship between cell growth and biosurfactant production. Similar results were reported by other investigators (21,32,33) not only with B. subtilis strains, but also with other species of bacteria. The... 

Furthermore, Fox and Bala (23) verified that although the addition of yeast extract could stimulate growth of a B. subtilis strain in an established potato medium, it did not decrease surface tensions. In fact, it has previously been related that the higher growth did not translate into lower surface tensions (24). In reality, the reduction in surface tension is inversely related to cell growth. 

The experimental disinfectant solutions tested exhibited significant sporicidal activity against B. subtilis spores. The B. subtilis strain used in these evaluations is the same one specified in the AO AC sporicidal test. Spores from... 

In this experiment you will use DNA isolated from a wild-type (Trp+) strain of B. subtilis as described in Experiment 19 to transform a competent Trp- mutant strain of B. subtilis (strain 168) from tryptophan auxotrophy (Trp ) to tryptophan prototrophy (Trp+). The results will also enable you to determine the transformation efficiency obtained with this protocol. 

Stock culture of B. subtilis strain 168 (Bacillus Genetic Stock Center strain 1A1)... 

Recipient B. subtilis strain 168 cells in sterile saline... 

Recipient B. subtilis strain 168 cells in sterile saline— These cells are conveniently provided as thawed 0.6-ml aliquots of frozen competent cells prepared as described in Experiment 20. You will require approximately 250 ml of these cells. 

Another biological effect of tilorone, based on its specific interaction to DNA is a selective inhibition of sporulation of B. subtilis strain 60015. At a drug con-... 

In the amino acid family are a number of interesting cyclopropyl compounds including the bicyclic methanoproline (6), produced by S. zamoyceticus SF-1836 with antibacterial activity against Xanthomonas and B. subtilis strains. The compound is apparently a proline... 

The uptake of aluminum, cadmium, chromium, cobalt, cop-per, iron, lead, magnesium, manganese, molybdenum, nickel, silver, tin, and zinc by B. subtilis Strain 168 is reported. These data were obtained during the lag phase, exponential phase, stationary phase, and the sporulation phase of the maturation cycle of this bacterial strain. Nonflame atomic absorption spectrometry was the method of analysis for all the metals except calcium, which was determined by flame atomic absorption spectrometry. The complete microbiological and analytical procedures are described. Uptake curves as a function of moles per cell, of moles per dry weight of a cell, and of percent available are reported. The data show that these metals seem to be required for growth. No attempts were made to postulate the roles played by these metals. 

Furthermore, developments by the DeAngelis group were the bases of a new commercial process launched by Novozymes for the fermentative production of hyaluronic acid (see Brown et al [49]) using recombinant B. subtilis strains. 

Different bacteriophages have been used to transduce noncompetent B. subtilis strains. Detailed protocols for transduction with the PBSl and SPPl phages... 

To reduce the very high maintenance demand of the riboflavin-overproducing B. subtilis strain 1012 pRF93 a cydBC knockout mutant was constructed [243]. 

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