Injectable composites applications

Elution is one of the critical step for successful separation. Sample application in affinity chromatography is performed usually by injection or application in the presence of mobile phase which is prepared in appropriate pH, ionic strength and solvent composition for solute-ligand binding. This solvent is referred as application buffer [8]. In the presence of application buffer, compounds which are complementary to the affinity ligand will bind while the other solutes in the sample will tend to pass through the column as nonretained compounds. After... 

Depending on the application, different demands are placed on the melt viscosity of the polymer. For injection molding applications, lower melt viscosities are required than for extrusion applications. For a given comonomer composition and processing temperature, the melt viscosity of the cycloolefin copolymers increases with the mean molecular weight. 

Table 6.2 Injectable composites and applications in tissue engineering... 

The actual loading capacity always depends on the sample composition and the separation problem. As a rule the volume of the loaded sample should not exceed 5% of the column volume. However, this recommendation is valid only for preparative runs. For analytical applications when a high resolution is needed, the volume of the injected sample should be about 1% of the total column volume or even less. For a preparative run on a 1000 X 200-mm column (bed height 60 cm), two different sample volumes were injected. If the sample volume is 0.3% of the total bed volume, the separation is more efficient... 

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