Initial Selection of HPLC Column

Chapter 3 discusses the characteristics of columns and packing materials. Although there is a wide choice of RPC-bonded phases on the market, a silica- [Pg.199]

Sample type Analytes type Common mode [Pg.200]

Reversed-phase (RPC), ion-pair (IP), ion-exchange (IEC), hydrophilic interaction (HILIC), hydrophobic interaction (HIC), normal-phase (NP), gel permeation (GPC), size-exclusion (SEC), nonaqueous RP (NARP). [Pg.200]

Guidelines on mobile phase selection are discussed in Chapter 2, Section 2.3 and are illustrated in the case study below. After evaluation of the first sample chromatograms, further method development to fine-tune the separation will be performed until all method goals are achieved. Other bonded phases or column configurations can be selected to enhance method performance. These are discussed in the Section 8.6. [Pg.200]

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