Inhibitor method

Radzicka, A., and Wolfenden, R., 1995. Transition state and mnltisnbstrate analog inhibitors. Methods in Enzymology 249 284-312. 

Branchini, B. R. (2000). Chemical synthesis of firefly luciferin analogs and inhibitors. Method. Enzymol. 305 188-195. 

Volume 63. Enzyme Kinetics and Mechanism (Part A Initial Rate and Inhibitor Methods)... 

The mechanism of chain initiation in cyclohexanol by the reaction with dioxygen was studied by the inhibitor method [57]. It was established that... 

The rate of radical generation was measured by the inhibitor method and turned out to be [46-50]... 

As say s for the identification and evaluation of histone acetyltransferase inhibitors. Methods (San Diego, Calif), 26, 245-253. 

Rando RR (1977) Mechanism-based irreversible enzyme inhibitors. Methods Enzymol 46 28 11... 

Fabbro D, Cowan-Jacob SW, Mobitz H, Martiny-Baron G (2012) Targeting cancer with small-molecular-weight kinase inhibitors. Methods Mol Biol 795 1-34... 

Comparison of incubation methods The inhibitor methods using have advantages in higher sensitivity, smaller sample volume, easier analysis and therefore greater throughput than the tracer approaches. Therefore, in spite of the potential artifacts mentioned above, the inhibitor approach remains attractive to some investigators. Incubations of 24 h up to 12 days were used to compare the inhibitor, NH4+ oxidation and dilution methods to estimate nitrification... 

Tautz L, Mustelin T (2007) Strategies for developing protein tyrosine phosphatase inhibitors. Methods 42 250-260... 

Umezawa, K. Imoto, M. Use of erbstatin as protein-tyrosine kinase inhibitor. Methods EnzymoL, 201, 379-385 (1991)... 

Gubemator, K., Heinze-Krauss, I., Angehm, P., Chamas, R.L., Hubschwerlen, C., etal. Structure-based design of potent j3-lactamase inhibitors. Methods Princ. Med. Chem. 6 89-103. 

Loskutoff, D. J. and Schleef, R. R, 1988, Plasminogen activators and their inhibitors. Methods in Enzymology 163, 293-302... 

Melillo G. Hypoxia-inducible factor 1 inhibitors. Methods Enzymol 2007 435 385 02. 

The question of inhibitor penetration is one that is not yet fully resolved for any of the inhibitors. Methods of getting inhibitors into the concrete were discussed in Section 5.6. Obviously if the inhibitor is sprayed directly on to the rebar in a repaired area, or if applied into drilled holes or slots cut into the surface, or to a milled surface before installing an overlay then there will be more rapid access to the steel than for a simple application to the concrete surface. 

The so-called inhibitor method, based on a measurement of the rate of consumption of an inhibitor [32], is frequently used to determine the rates of initiation and development of chains during reactions of liquid-phase oxidation. The weak aspects of this method have been indicated in [33, 34]. 

MW, molecular weight variation method K, kinetic method I, inhibitor method C, ratoactive alkyl method T, tritiated quenching agent method CO, radioactive carbon monoxide method. 

Allison, R. D. and D. L. Purich, 1979. Practical considerations in the design of initial velocity enzyme rate assays, in Methods in Enzymology, Vol. 63, Enzyme Kinetics and Mechanism, Part A, Initial Rate and Inhibitor Methods (D. A. Purich, Ed.), pp. 3-22, Academic Press, San Diego, CA. 

Pippard D. A. (The British Petroleum Company Limited) (1983). Corrosion inhibitors, method of producing them and protective coatings containing them. US patent application 4405493. 1983-Sept-20. 

Effects of catalyst inhibition and activation are substantial for regulating their activity in complicated, in particular, physiological, systems. Inhibitor methods serve as a tool for studying the structure and mechanism of the catalytic effect of active sites. Below we describe the most often met types of inhibition and activation. The graphical methods for analysis of kinetic data are illustrated in Fig. 14.2. 

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