Indicators of Toxicity in Cultured Cells

Routine observation of cultured is usually carried out by phase contrast microscopy, utilizing the inverted phase contrast microscope. More recently, more detailed observations have become possible utilizing fluorescent tags and inverted fluorescent microscopes. Fluorescent tags currently in use permit the assessment of oxidant status and mitochondrial function as well as the intracellular concentration of sulfhydryl groups, Ca2+,H+,Na+, andK+. [Pg.14]

Toxicity to cultured cells may be the result either of inadequacies in the culture or the toxicity effects of the chemical being investigated. Short-term toxicity is usually [Pg.14]

Cell Line Source Differentiated Cell Type Toxicant Measured End Point [Pg.15]

N1E-115 Mouse neuroblastoma Cholinergic neuron Lead Pyrethroid insecticide Blockage of voltage-dependent Ca2+ channels Prolonged open time for voltage-dependent Na+ channels [Pg.15]

PC12 Rat pheochromocytoma (adrenal medullary tumor) Adrenergic neuron Tricresyl phosphate (organophosphate) Inhibition of neurofilament assembly and axonal growth [Pg.15]