Incubation procedure

Numerous workers have found that measurements of serum lipase activity are useful in the diagnosis of pancreatitis (83, 84, 85). Despite this, serum lipase determinations are not usually performed in clinical laboratories, probably due to inherent problems associated with the conventional methods, based on an emulsified lipid substrate. The methods are also not very suitable for manual batch analysis nor for automation due to laborious post incubation procedures. 

The reconstitution of active E. coli 50 S subunits, in contrast to that of 50 S particles from B. stearothermophilus (Nomura and Erdmann, 1970), requires a two-step incubation procedure (Nierhaus and Dohme, 1974 Dohme and Nierhaus, 1976). The assembly process occurs in four steps from 23 S RNA to 50 S particles, leading to formation of 33 S, 41 S, and 48 S intermediates. The step from 33 S to 41 S consists of a compact folding of the 33 S intermediate, without addition to any protein component. This drastic conformational change has been demonstrated by biochemical and electron-microscopic studies (Sieber and Nierhaus, 1978 Sieber et al, 1980 Nierhaus, 1982). Kinetic analyses performed at... 

Figure 5. SDS-PAGE of a reconstituted moiwoxygermse system from rat liver that had been labeled with 5 by incubation with parathion. The incubation procedures were essentially as described in Figure 4 (20).

It is this sort of mechanism that I think must underlie the incubation procedure for lucid dreaming. By putting the notebook at my bedside... 

As the degree-of-activation of mitochondria is said to influence their physical appearance, it may be suggested that some of the observed unmasking phenomena may be due to the actual state of the brown fat mitochondria at the time of isolation, and/or to the incubation procedure, rather than reflecting true changes in the in situ qualities of thermogenin itself. This, however, remains to be investigated. 

Chapman,R.A., Harris, C.R. unpublished results) provided an opportunity to observe the persistence of enhanced activity in the absence of further treatment. E-factors observed at times ranging from 52 to 164 weeks following the third annual treatment are listed in Table IX. Soils previously treated with carbofuran, isofenphos and trimethacarb were tested by the aqueous incubation procedure mentioned previously (16) and are assessed as either active or slightly active. 

To test the effect of solarization in the field on both the generation of enhanced activity and the activity of enhanced microbial populations, microplots containing clay loam were treated with granular carbofuran. Some plots were covered with plastic immediately (July 6) and the remainder were left uncovered. Soil samples were taken at intervals from all plots and tested for enhanced activity using our previously developed aqueous incubation procedure(16). Table XII shows the results of some of these tests. The times required for 50% of the initial 10 ppm of carbofuran to disappear from the test solution (DT/50) are a measure of the enhanced activity in the soil (DT/50 > 8 days, not active < 5 days, active). Enhanced activity was generated in the uncovered plots... 

Incubation Incubation procedures of test compoxmd in human or rat liver microsomes in the presence of GSH or NAc or KCN and NADPH... 

Slides are incubated with antibody in cytomailers (each holding five slides and approx 10 mL incubation volume) to conserve materials and ensure uniformity in incubation procedures. Sections should be mounted towards the bottom of the slides to conserve buffers and antibody required for various incubations. 

However, there is no standard yet concerning size, design, and type of in vitro test systems for blood compatibility (hemocompatibility). In consequence, a wide variety of different test systems are currently applied in the development of new materials. As the dependence of the in vitro alteration of blood on several experimental conditions is rather complex, the comparison of results from hemocompatibility tests performed in different setups and by different incubation procedures remains ambiguous and may even lead to contradicting conclusions depending on the type of experimental approach used [66]. 

We have recently examined the responsiveness to Mg + and MgATP of the subunit aggregates from a superactive form of PRPP synthetase with normal substrate and inhibitor binding properties but an increased maximal reaction velocity. The distribution of forms of the mutant enzyme on sucrose gradient after the dilution and incubation procedures described above was indistinguishable from that of normal PRPP synthetase. In several respects, however, the mutant enzyme behaved in an abnormal fashion. First, under conditions of suboptimal Mg2+... 

The Incubation procedure for l- c stearic acid desaturation was performed as previously described (De Tomas et al., 1973) except that 25 mg of soluble protein (100,000 xg supernatant) was added to the incubation solution. 

Control experiments were also performed using the protein with the chromophore detached by hydroxylamine, inserted into the lipid bilayer with the same incubation procedure employed for the photopigment. Light-independent current transitions between two constant levels, corresponding to conductance jumps of about 500 pS were exhibited by these membranes. 

From the labeling pattern of CF1 with eosin-SCN two states of the enzyme could be discriminated, the latent and the active state. When membrane bound CF1 was incubated with eosin-SCN (100 urn) at pH 8.3 for three minutes without energization of the membrane, we found 1 mol of bound eosin-SCN/mol CF1 bound to the alpha-subunit of CF1. This neither impaired the Ca -ATPase activity of isolated CF1 nor its ability to reconstitute cyclic photophosphorylation in depleted membranes. If the same incubation procedure was performed after one-minute illumination of chloroplasts under conditions of cyclic electron transport, CF1 contained 2 moles of eosin-SCN/mol CF1. One of the label mol cules was bound to the alpha- and another one to the beta-subunit. The Ca -ATPase of isolated CF1 was inhibited to 50 of the control, whereas the ability of labeled CF1 to reconstitute cyclic photophosphorylation in depleted membranes was not affected. This extra binding of one eosin-SCN into the beta-subunit of CF1 during light incubation of chloroplasts was abolished by uncoupler and could be prevented by the presence of 5mM ATP during incubation. 

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