In vitro pancreatic digestion

Table III. Effect of In Vitro Pancreatic Digestion pH on Carbohydrate and Protein Digestion and Calcium Solubility...

Table II. Effect of Peptic and Pancreatic Enzymes on Mineral Solubility After In Vitro Digestion...

During in vitro glycemic analysis of freshly boiled minced potatoes of a range of varieties nearly all of the starch was almost immediately digested by pancreatic amylase. Not surprisingly, therefore, in vitro analysis has not revealed any effects of storage time in potatoes stored for 1-3 months or 8-10 months (Lehman et al, 2005). 

Mun, S., Decker, E.A., McClements, D.J. (2007). Influence of emulsifier type on in vitro digestibility of lipid droplets by pancreatic lipase. Food Research International, 40, 770-781. 

In another set of studies, it has been reported that the in vitro digestibility of lipid droplets by pancreatic lipase is significantly affected by emulsifier type (Mun et al, 2006, 2007 Park et al., 2007). Intuitively, one might expect that a thick dense layer of strongly bound protein-polysaccharide complex at the oil-water interface would reduce considerably the in vivo accessibility of lipases, and hence would reduce the rate of human metabolism of fats. Establishment of the validity of this hypothesis must still await consolidation of a substantial body of detailed results from independent systematic studies on a broad range of mixed biopolymer systems. 

Borgstrom, B. 1980. Importance of phospholipids, pancreatic phospholipase A2, and fatty acid for the digestion of dietary fat In vitro experiments with the porcine enzymes. Gastroenterology 78, 954-962. 

Bemback, L fiiacabcig, and G. Hcmcli. The compieie digestion of human milk triacylglyccxol In vitro requires gastric lipase, pancreatic colipase-depcndent lipase and bile salt-stimulated lipase. J. Clio. Invest. Jf5 1221 (1990). 

M. UndsttOm, B. Stentby, and B. BotgstrtJrn. Conceited notion of human carboxyl ester lipase and pancreatic lipase during lipid digestion In vitro Impair-... 

Bernback, S., Blackberg, L., Hernell, O. 1989. The complete digestion of human milk triacyl-glycerol in vitro requires gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase.. / Clin. Invest. 85, 1221-1226. 

A second approach is to subject the food to a simulated gastric or gastrointestinal digestion using purified peptic and/or pancreatic enzymes with subsequent measurement of the soluble iron released by the digestion (10-13). Advantages of in vitro methods include 1. their low cost and speed, 2. their reduced... 

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