In preparative HPLC

S. Miller, Prep LC Systems for Chemical Separations," Anal. Chem. 2003, 75. 477A R. E. Majors, The Role of the Column in Preparative HPLC, LCGC 2004,22, 416 A. Brandt and S. Kueppers, Practical Aspects of Preparative HPLC in Pharmaceutical Development and Production, LCGC 2002,20, 14. 

Although classical column chromatography over chiral stationary phases has been used for several years, HPLC has until recently been regarded mainly as an analytical tool. However, methods have now been developed and many papers have been published on the various applications of particular stationary phases in preparative HPLC, which may be the method of choice when the preparation of small amounts of material are required for screening purposes, for use in biological assays, or as standards in purity assays. The use of chromatographic techniques may be quicker and less risky than custom synthesis and resolution and can guarantee material to a defined specification. 

Although they are extremely useful analytically, the protein based stationary phases 3-6,371 have found little application in preparative HPLC because they suffer from low loading capacity, due primarily to the low number of active sites. The natural macrocylic molecules Cyclodextrin 3 8,3 91 and antibiotics such as Vancomycin 3 10] have shown some promise. Synthetic chiral crown ethers 311 are particularly useful for the separation of chiral primary amines. 

X. EFFECT OF MASS TRANSFER RESISTANCES IN PREPARATIVE HPLC OF POLYPEPTIDES AND PROTEINS... 

X. Effect of Mass Transfer Resistances in Preparative HPLC of Polypeptides and Proteins 178... 

Analysis of Drugs in Preparations HPLC has found widespread use for the quantitative analysis of drugs in preparations of pharmaceutical and illicit manufacture. Drug concentrations are generally high enough to allow dissolution of the sample (tablet, powder, ointment, etc.) in a suitable solvent followed by injection. Ultraviolet-visible, fluorescence, or refractive index detection is normally used. 

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