Implementation of Multidimensional TCSPC

To summarise, the lifetime teehnique for a laser seanning microscope should  

This is exactly what advanced multidimensional TCSPC is capable of. The scanning technique of advaneed TCSPC (see page 37) therefore almost perfectly fits the laser seanning mieroseope. 

A second requirement is that the scan clocks (i.e. a frame clock, a line clock, and a pixel clock) are available from the scan controller of the microscope. This is rarely a problem in modem scanning microscopes, which usually make the scan clocks available through an external connector. 

A relatively simple way to build a lifetime microscope with descanned detection is to use a fibre output from the scanning head. Fibre outputs are available for the Biorad Radianee 2100, and for the Zeiss LSM 510 NLO and LSM 510 Meta systems. The required connections are shown in Fig. 5.73. 

The fluorescence light from the sample is delivered to the detector via the fibre output from the scan head. Often an additional laser blocking filter is required in front of the detector (see Sect. 5.7.7, page 154). 

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