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Immunostaining intensity sections

As described below, ROD values are used to compare the same cellular type from different tissue sections after experimental treatments. In this way, the immunostaining intensity of an antigen can be measured. [Pg.94]

Immunoreactivity diminished or destroyed on pre-cut tissue sections. The intensity of immunostaining may be diminished when pre-cut tissue sections are exposed to air. Use freshly cut sections and reseal paraffin-embedded blocks. 29-33... [Pg.139]

The use of the MEl antibody was initially reported by O Hara and colleagues in 1990. " MEl is a monoclonal antibody generated from the mesothelial cell line SPClll and reacted with normal mesothelial cells and malignant epithelial mesotheliomas. Their antibody was only useful on frozen section tissue and showed immunostaining of 40 of 40 (100%) epithelial mesotheliomas. Nineteen well and moderately differentiated primary pulmonary adenocarcinomas failed to stain with the MEl antibody, but one poorly differentiated pulmonary adenocarcinoma showed intense immunostaining. [Pg.427]


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See also in sourсe #XX -- [ Pg.33 , Pg.34 , Pg.35 , Pg.36 , Pg.37 ]

See also in sourсe #XX -- [ Pg.33 , Pg.34 , Pg.35 , Pg.36 , Pg.37 ]




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