Immunosensors dendrimers

Based on preliminary photophysical end biological investigations a conclusion can be taken that the dendrimer membrane possesses promising properties for applying in biosensor and immunosensor constructions with fluorescent detection (Grabchev et al. 2007b) (Fig. 3). 

Kwon, S. J. Kim, E. Yang, H. Kwak, J. An electrochemical immunosensor using ferrocenyl-tethered dendrimer. Analyst 2006, 131,402-406. 

In its simplest, QCM, format, protein-aptamer interactions were analyzed by Liss et al. (2002). They compared the interaction of IgE with DNA aptamer as well as with anti-IgE antibodies. Although the detection limit was similar in the two cases, the advantage of the aptasensor was its possibility of surface regeneration, which was impossible for an antigen-based biosensor. However, recently it has been shown that immobilization of anti-IgE on the dendrimer surface also allows us to regenerate an immunosensor (Svobodova et al., 2006). The QCM method was recently compared with the electrochemical biosensor assay of thrombin detection (Hianik et al., 2005, 2007). It has been shown that the sensitivity of thrombin detection was similar for the two methods. Mascini and co-workers showed that similar results in sensitivity and selectivity in the detection of Tat peptide with RNA aptamer can be obtained by the QCM and SPR methods (Tombelli et al., 2005b). 

Kim, D.-M. (2010) Md.A. Rahman, M.H. Do, C. Ban, Y-B. Shim, An amperometric chloramphenicol immunosensor based on cadmium sulfide nanoparticles modified-dendrimer bonded conducting polymer. Biosens. Bioelectron, 25, 1781-1788. Conzuelo, F., Gamella, M., Campuzano, S. et al. (2012) Disposable amperometric magneto-immunosensor for direct detection of tetracyclines antibiotics residues in milk. Anal. Chim. Acta, 737, 29-36. 

Lin MH, Liu YJ, Yang ZH, Huang YB, Sun ZH, He Y, Ni CL (2012) Construction of sensitive amperometric immunosensor based on poly(amidoamine) dendrimer and one-step iraiic-liquid-assisted graphene/chitosan platform for benzo[a pyrene detection. Int J Electrochem... 

Thin hydrogel films made of ferrocene-modified poly(ethylene imine) with embedded enzymes can act as biosensors for glucose [600, 601]. Similarly, other glucose-dependent biosensors have been constructed [602-604] e.g., a poly (vinylferrocene)-enzyme composite deposited on an electrode acted as a mediator for glucose oxidation [605-610]. Immunosensors were realized on glassy carbon electrodes by ferrocene-dendrimer-modified antibodies, which quench electrochemiluminescence [611]. 

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