Enzyme embedded

Tricalcium phosphate was also used as an enzyme embedding matrix. Das and coworkers [229] demonstrated that acid phosphatase and amylase immobilized on Ca3(P04)2 retained their activities with increased thermal stability. 

Further progress in understanding the mechanism of action and kinetics of immobilized enzymes will require the study, both theoretical and experimental, of more complex enzyme systems. Of particular interest will be the study of the highly specific interactions between an enzyme embedded in a native membrane and the adjacent high or low molecular weight compounds. 

Catalysis occurring in a single phase. Most enzymes are homogeneous catalysts however if an enzyme embedded in a solid matrix acts on soluble substrates, aspects of heterogenous catalysis become important. See Heterogeneous Catalysis... 

Water-insoluble enzymes have now been developed very few references bear dates prior to 1960, and yet the overall principle of attachment of an enzyme to an insoluble matrix is simple and simulates the natural mode of action and environment of enzymes embedded in biological membranes. The insolubilization of enzymes with retention of activity has already made an impact on the chemistry of biological molecules and systems, and, for an essentially new-born subject, a> large number of reviews dealing with their preparation have been published. " ... 

The actual experimental conditions employed range from the use of cultured broths of common microorganisms, through the use of cell-free extracts, to the use of columns of fully immobilized enzymes embedded on cellulose triacetate fibres, and reactions are rapid at 30°. 

Enzyme-Embedded LbL Devices 3.1 Thin Film Type... 

A gamut of physicochemical and biochemical techniques have demonstrated that succinate, coenzyme Qio, and ATP activate succinate dehydrogenase, and these modulations are much more effective on enzymes embedded in intact mitochondria than on particulate of partially solubilized preparations [98]. On the basis of these observations, a tentative hypothesis for succinate dehydrogenase regulation has been proposed. The main purpose of the enzyme is to remove the... 

The enzymes embedded within the reverse micelles retain full enzymic activity it is noteworthy, however, that many of them are found to undergo an increase in conformational rigidity , i.e., in the percentage of secondary structure (e.g. helix content), and that the effect is more marked the lower the water content of the micellar system. The special microenvironment in which biopolymer molecules are enclosed might therefore exert a marked effect on their conformation, stability and activity. 

Finally, I should like to point out that it might be possible to use enzymes embedded within a biological membrane as probes for detecting the nature of the membrane-environment surrounding the enzyme. 

Copolymerization of periodate-oxidized cellulose with ycidyl methacrylate in the presence of the enzyme Embedding in a polyacrylamide gel by way of photo-induced polymerization of acrylamide... 

Enzymes embedded in a cross-linked redox hydrogel film. 

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