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Immunosensors cross-reactivity

Two immunosensors developed by O Regan et al. [89,90] have demonstrated their usefulness for the early assessment of acute myocardial infarction (AMI). Human heart fatty-acid binding protein (H-FABP) is a biochemical marker for the early assessment of AMI. The authors constructed an amperometric immunosensor for the rapid detection of H-FABP in whole blood. The sensor is based on a one-step, direct sandwich assay in which the analyte and an alkaline phosphatase (AP) labelled antibody are simultaneously added to the immobilized primary antibody, using two distinct monoclonal mouse anti-human H-FABP antibodies. The substrate p-amino-phenyl phosphate is converted to p-aminophenol by AP, and the current generated by its subsequent oxidation at +300 mV vs. Ag/AgCl is measured. The total assay time is 50 min, and the standard curve was linear between 4 and 250 ng ml . The intra- and inter-assay coefficients of variation were below 9%. No cross-reactivity of the antibodies was found with other early cardiac markers, and endogenous substances in whole blood did not have an... [Pg.559]

Pseudohomogenous amperometric displacement immunosensors have also been developed. The displacement assay is performed on a disposable screen printed carbon electrode and takes advantages of the cross-reactivity of some monoclonal antibodies. As an example, the monoclonal anti-2,4-D antibody exhibits a relative cross-reactivity toward immobilized MCPA. In the presence of the target analyte (2,4-D) a displacement effect is observed. Consequently, the peroxidase label of the remaining anti-2,4-D antibodies is detected at the carbon electrode, when adding hydrogen peroxide and potassium iodide as redox mediator, at OV versus Ag/AgCl. [Pg.2074]

PAHs are always found as a mixture of individual compounds with similar molecular structure, similar electron density, and a lack of side groups. Therefore, an immunosensor based on SPEs was developed for PAH detection using a co-exposure competition assay. The immunosensor displayed cross-reactivities of varying degrees towards 16 important PAH compounds. ... [Pg.180]


See other pages where Immunosensors cross-reactivity is mentioned: [Pg.519]    [Pg.143]    [Pg.168]    [Pg.563]    [Pg.82]    [Pg.25]    [Pg.29]    [Pg.260]    [Pg.6]   
See also in sourсe #XX -- [ Pg.233 ]




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