Immunogold electron microscopy

Tao-Cheng, J. H. and Zhou, F. C. Differential Polarization of serotonin transporters in axons versus soma-dendrites an immunogold electron microscopy study. Neuroscience 94 821-830, 1999. 

Ohno M, Takemura G, Ohno A, Misao J, Hayakawa Y, Minatoguchi S, et al. Apoptotic myocytes in infarct area in rabbit hearts may be oncotic myocytes with DNA fragmentation Analysis by immunogold electron microscopy combined with in situ nick end-labeling. Circulation 1998 98 1422-1430. 

Godsave SF, Wille H, Kujala P, Latawiec D, DeArmond SJ, Serban A, Prusiner SB, Peters PJ (2008) Cryo-immunogold electron microscopy for prions toward identification of a conversion site. J Neurosci 28 12489... 

Hemphill, A., Seeback, T. and Lawson, D. (1991) The Trypanosoma brucei cytoskeleton ultrastructure and localization of microtubule-associated and spectrin-like proteins using quick-free, deep-etch, immunogold electron microscopy. J. Struct. Biol. 107 211-220. 

Mapping Proteins to Nuclear Pore Complexes by Immunogold Electron Microscopy... 

A frequently used and particularly successful method for localizing nucleoporins to the NPC is immunogold electron microscopy. The principle of the method is to raise antibodies against specific nucleoporins, to react permeabilized cells, isolated nuclei, or histological sections with these primary antibodies, and to visualize the subcellular binding sites of the antibodies by electron microscopy. Of course, antibodies can be only detected by electron microscopy if they are marked by an electron-dense label. For that purpose nanometer-sized gold particles are attached to the primary antibodies either directly or indirectly. 

Grote, M., Kubitscheck, U., Reichelt, R., and Peters, R. (1995). Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopy. J. Cell Scl 108, 2963-2972. 

---