Immunocytochemical localization nervous system

Araki, T., Yamano, M., Murakami, T. el al. (1988). Localization of glycine receptors in the rat central nervous system an immunocytochemical analysis using monoclonal antibody. Neuroscience 25, 613-24. 

Sari, Y., Miquel, M. C., Brisorgueil, M. J. et al. (1999). Cellular and subcehular localization of 5-hydroxytryptamineiB receptors in the rat central nervous system immunocytochemical, autoradiographic and lesion studies. Neuroscience 88, 899-915. 

Mensah-Niagan AG, Feuilloley M, Dupont E, et al Immunocytochemical localization and biological activity of 3 (3-hydroxysteroid dehydrogenase in the central nervous system of the frog. J Neurosci 14 7306-7318, 1994... 

Brownlee, D.J.A. and Fairweather, I. (1996) Immunocytochemical localization of glutamate-like immunore-activity within the nervous system of the cestode, Mesocestoides cord and the trematode, Fasciola... 

Expression of the PBAN mRNA has been investigated in the central nervous system in B. mori (Sato et al., 1993, 1994), H. zea (Ma et al., 1998), and H. assulta (Choi et al., 1998a). In B. mori, in situ hybridization revealed a total of 12 positive cells which were localized as a cluster at three distinct regions along the ventral midline of the SEG (Sato et al., 1994). Similar clusters of neurons were localized in H. zea, and the PBAN encoding gene was shown to be expressed predominantly in the SEG and at relatively low levels in other parts of the CNS (Ma et al., 1998). These findings are similar to the immunocytochemical evidence presented in section 5.2.2. 

Kia HK, Miquel MC, Brisoigueil MJ, et al. Immunocytochemical localization of serotonin1A receptors in the rat central nervous system. J Comp Neurol 1996 365 289-305. 

Alonso G, Phan V, Guillemain I, Saunier M, Legrand A, et al. 2000. Immunocytochemical localization of the sigma(l) receptor in the adult rat central nervous system. Neuroscience 97 155-170. 

Finley JCW, Maderdrut JL, Roger LJ, Petrusz P (1981) The immunocytochemical localization of somatostatin-containing neurons in the rat central nervous system. Neuroscience <5 2173-2192. 

Merchenthaler I, Maderdrut JL, Altschuler RA, Petrusz P (1986b) Immunocytochemical localization of proenkephalin-derived peptides in the central nervous system of the rat. Neuroscience 17 325-348. 

Numerous tests carried out in insects with antibodies raised against mammalian neuropeptides revealed reaction products within and outside of the nervous system. Conversely, certain neuropeptides first identified in invertebrates were shown to occur also in mammals and other vertebrates. These commonalities are indicative of a long evolutionary history, as well as a wide distribution of active neuropeptides in neural and non-neural tissues. One of the new insights gained from the use of multiple antisera and carefully conducted specificity tests was the immunocytochemical detection in the insect brain of molecules closely resembling mammalian ACTH, prolactin, and insulin,. The localization of these substances suggests a neurotransmitterlike or neuromodulatory rather than a hormonal role. 

Merchenthaler I, Csemus V, Csontos C, Petrusz P, Mess B. 1988. New data on the immunocytochemical localization of thyrotropin-releasing hormone in the rat central nervous system. Am J Anat 181 359-376. 

Merchenthaler I, Liposits Z, Reid JJ, Wetsel WC (1993) Light and electron microscopic immunocytochemical localization of PKCJ immunoreactivity in the rat central nervous system. J. Comp. Neurol., 336, 378-399. 

N ssel, D. R. (1996) Advances in the immunocytochemical localization of neuro-active substances in the insect nervous system. J. Neurosci. Methods, in press. Cuello, A. C., ed. Immunohistochemistry. IBRO Handbook Series Methods in Neurosciences, vol. 3, Wiley, Chichester, UK. 

For immunocytochemical localization of certain amino acid transmitter enzymes in the central nervous system, a good preservation of tissue morphology and antigen antigenicity is essential In general, perfusion fixation is better than immersion fixation. For perfusion fixahon one may perfuse the fixative into the animal circulatory system through the left ventricle of the heart or through the abdominal aorta. 

Skiebe, P., Dietel, C., Schmidt, M. (1999) Immunocytochemical localization of FLRFamide-, proctoKn-, and CCAP-like peptides in the stomatogastric nervous system and neurohemal structures of the crayfish, Cherax destructor. J Comp Neurol, 414, 511-532. 

Lee H.M. and Wyse G.A. 1991. Immunocytochemical localization of octopamine in the central nervous system of Limulus polyphemus A light and electron microscopic study. /. Comp. Neurol. 307 683-694. 

---