Immunoassay species-specific proteins detection

Both methods have their advantages, disadvantages, and commonalities. The principal advantage of immunoassays over DNA assays is the fact that they detect the actual component of interest (i.e., food allergens, mycotoxins, and species-specific proteins used in speciation, among others), whereas DNA is used only as a marker for the presence of these components in food ingredients or processed foods. Further comparisons between the two are described below. 

The other small protein markers of GFR are detected by immunoassays with reagents specific for the human proteins the cross-reactivity of these reagents with other species and the usefulness of these markers in animal models have not been well established (Loeb 1998). 

Table. 1 displays the results to detect deactivated SARS-CoV and other viruses, it showed absolutely detectability to all of SARS-CoV species, while no cross reaction to other virus, especially to common human coronal Virus (HCoV-OC43 and HCoV-229E) was found. So the sensitivity and specificity of this immunoassay was fully acceptable. According to the data obtained from US CDC strain, its detectable limit was nearly 3 copies of virus per 50 [iL test. This extraordinary high sensitivity seemed to indicate the additional N protein that much more than whole virus was excreted in SARS-CoV culture. Nevertheless it seems that the N protein is a sensitive marker for SARS-CoV proliferation. 

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