Immuno toxins

Lambert, J.M., Senter, P.D., Yau-Young, A., Blattler, W.A., and Goldmacher, V.S. (1985) Purified immuno-toxins that are reactive with human lymphoid cells Monoclonal antibodies conjugated to the ribosomeinactivating proteins gelonin and the pokeweed antiviral proteins./. Biol. Chem. 260, 12035-12041. 

The minimum antibody binding domain, Fv, has also been linked to toxin to produce a more compact, recombinant immuno-toxin. The recombinant F -toxin appears to be more stable and exhibits less liver accumulation [27,28]. The immunogenicity of Fv-toxin as a consequence of the highly antigenic determinants of bacterial toxin remains a barrier to development. 

Rastan, I.I., and R.I Kreitman, Immuno-toxins for targeted cancer therapy. Adv Drug Deliv Rev, 1998. 31(1-2) 53-88. 

Engert A, Diehl V, Schnell R et al. (1997). A phase-I study of an anti-CD25 ricin A-chain immuno-toxin (RFT5-SMPT-dgA) in patients with refractory Hodgkin s lymphoma. Blood, 89, 403-410. 

Francisco JA, Gawlak SL, Miller M, et al. Expression and characterization of bryodin 1 and a bryodin 1-based single-chain immuno-toxin from tobacco cell culture. Bioconjug. Chem., 1997 8(5) 708-713. 

Casellas, P, Dussossoy, D, Falasca, AI, Barbieri, L, Guillemot, JC, Ferrara, P, Bolognesi, A, Cenini, P, Stirpe, F Trichokirin, a ribosomeinactivating protein from the seeds of Trichosanthes kirilowii Maximowicz. Purification, partial characterization and use for preparation of immuno toxins. Eur. J. Biochem. 176(3), 581-588 (1988)... 

Burrows FJ, Thorpe PE. Eradication of large solid tumors in mice with an immuno-toxin directed against tumor vasculature. Proc Natl Acad Sci USA 1993 90 8996-9000. 

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