Immuno-like assays

Keywords Biomimetic assay Immuno-like assay Microchip Molecularly imprinted polymer... 

Advantages and Disadvantages of MIPs in Immuno-Like Assays. 120... 

Applications of Molecularly Imprinted Polymers in Immuno-Like Assays.135... 

Fig. 6 Optimization of the concentration of imprinted polymer in immuno-like assays. The plot represents the differences (%) observed in the binding of 250 nmol L-1 PAAP to the polymer when incubated in the absence and in the presence of 426 pmol L-1 of penicillin G. Binding solvent acetonitrile-water (99 1, v/v), T = 25 °C, incubation time 7 h n = 9)...

Table 1 Comparison between the figures of merit of selected immunoassays and immuno-like assays for the analysis of environmental and food samples... 

Table 3 Examples of radio immuno-like assays ...

Surugiu et al. [67] have introduced an Enzyme Immuno-Like Assays (EzILA) for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The label was a 2,4-D conjugate with the tobacco peroxidase (TOP) enzyme, which allows for both colorimetric and chemiluminescent detection. In this case, the polymer imprinted with 2,4-D was synthesized in the form of microspheres. In contrast, despite its higher binding capacity for radiolabeled 2,4-D, a conventional MIP prepared by bulk polymerization showed only weak binding of the 2,4-D-TOP tracer. 

Like in RILAs, an advantage of fluorescence detection is the possibility of developing homogeneous FILAs using direct or indirect (competitive or displacement) approaches. The fluorescence polarization immunoassay (PFIA) and their homolog fluorescence polarization immuno-like assay (PFILA) are two of the most widely used procedures in homogeneous fluoroassays. Both are based on the principle that fluorescence polarization gives a direct measure of the bound/free ratio of the labeled analyte (tracer) without the need for their separation [23, 28]. 

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