Tobacco peroxidases

TOP Tobacco peroxidase, HRP Horseradish peroxidase, APB A 3-Aminophenylboronic acid monohydrate, TBA 3-Thiopheneboronic acid, VYP 4-vinylpyridine, TRIM Trimethylolpropane trimethacrylate... 

Surugiu et al. [67] have introduced an Enzyme Immuno-Like Assays (EzILA) for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The label was a 2,4-D conjugate with the tobacco peroxidase (TOP) enzyme, which allows for both colorimetric and chemiluminescent detection. In this case, the polymer imprinted with 2,4-D was synthesized in the form of microspheres. In contrast, despite its higher binding capacity for radiolabeled 2,4-D, a conventional MIP prepared by bulk polymerization showed only weak binding of the 2,4-D-TOP tracer. 

Castillo J, Ferapontova E, Hushpulian D et al (2006) Direct electrochemistry and bioelectrocatalysis of H202 reduction of recombinant tobacco peroxidase on graphite. Effect of peroxidase single-point mutation on Ca2+-modulated catalytic activity. J Electroanal Chem 588 112-121... 

Recently, an enzyme-labelled assay for 2,4-D has been presented [19]. The label was 2,4-D conjugated to the enzyme tobacco peroxidase, which allowed for both colorimetric and chemiluminesence detection. In this instance, the 2,4-D MIP was synthesised in the form of microspheres [20]. In contrast, despite its higher binding... 

Having established the selectivity of the microspheres for 2,4-D, the template was then conjugated to tobacco peroxidase (TOP). Briefly, TOP was activated using NaI04 then coupled with diaminopropane. 2,4-D was converted to its N-hydroxysuccinimide ester and allowed to react with the derivatised TOP. After purification, the final 2,4-D/TOP ratio was estimated to be 8/1. 

Schepartz, A.I. Method for the determination of catalase activity in tobacco Tob. Sci. 18 (1974) 52-54. Schepartz, A.I. and D.G. Bailey Enzymatic activity in harvested cigar-fiUer tobacco Peroxidase and catalase Tob. Sci. 18 (1974) 105-107. 

D using the same monomer and TRIM as the cross-linker [28,29]. In this case, tobacco peroxidase was used as the enzyme and the chemiluminescent reaction with luminol was used for the detection. Infrared evanescent wave spectroscopy was also used to study 2,4-D imprinted polymers prepared using 4-VPy and MMA as the... 

Figure 14 Schematic presentation of ELISA-analogous sensor based on MIPs. 2,4-D had a competitive binding with tobacco peroxidase labeled 2,4-D, which was analyzed by chemiluminescence imaging.

PNP, peanut peroxidase TOP, tobacco peroxidase SPP, sweet potato peroxidase recHRP, recombinant wild type (WT) HRP. HRP PhelllTrp, WLP AsnJOVal, HRP AsnJOAsp, are HRP mutants. 

F.-D. Munteanu, L. Gorton, A. Lindgren, T. Ruzgas, 1. Emneus, E. Csoregi, 1. G. Gazaryan, 1. V. Ouporov, E. A. Mareeva, L. M. Lagrimini, Direct and Mediated Electron Transfer Catalyzed by Anionic Tobacco Peroxidase. Effect of Calcium Ions. Appl. Biochem. Biotech., 84 (2000) 1-13. 

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