Immobilization systems loading capacity

Another interesting modification of glass surfaces was introduced by Beier and Hoheisel.23 They synthesized a flexible, dendritic linker system that enables covalent immobilization of oligonucleotides and PNAs with high loading capacity in a controlled manner. This method facilitates the modulation of surface properties such as hydrophobicity and charge. The synthesis of the linker system involves two consecutive reactions an acylation of surface-bound amine groups with acid chloride (4-nitrophenyl-chloroformate or acryloylchloride) and subsequent reaction with an amine. A bis-amine results in a linker system, while a polyamine produces a dendritic structure (Fig. 14.3). Because polyamines possess primary and secondary amine... 

Recent years have witnessed the implementation of novel techniques for enzyme immobilization and entrapment aimed at increasing enzyme stabilization, enzyme activity, loading capacity, reusability, and easy separation of the bio-catalyst from the reactants and/or product. Microwave-irradiation [43], photo immobilization technology [44], enzymatic immobilization of enzymes [45, 46], and magnetic nanocarriers as supports are some of the new approaches that are currently being focused on. Recent advances in nanotechnology have led to the development of various nanostructured materials, which have provided with an inexpensive and efficient support system for the immobilization of enzymes. Surface chemistry [47] and different physio chemical... 

Figure 1. Capacity of model immunosorbent systems as a function of cycle number. (A) Goat anti-human IgC Fab fragment immobilized at 7 g/L. Column volume = 6 ml. (B) Same as above except that the initial antibody loading was 1 gL- and the immunosorbent volume was 4.2 ml.

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