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Toxin thiolation using 2-iminothiolane

Another way of utilizing SPDP is to again activate the antibody to create the pyridyl disulfide derivative, but this time thiolate the toxin component using 2-iminothiolane (Chapter 1,... [Pg.836]

Concentrate the thiolated toxin to lOmg/ml using centrifugal concentrators. Immediately use the modified protein in the conjugation reaction to prevent inactivation of 2-iminothiolane-modified molecules by recyclization (Chapter 1, Section 4.1). [Pg.840]

Figure 21.13 Sulfo-SMCC may be used to activate antibody molecules for coupling to thiolated toxin components. An intact A-B toxin molecule can be modified to contain sulfhydryls by treatment with 2-iminothiolane. Thiolation with this reagent retains the cytotoxic properties of the toxin while generating a sulfhydryl for conjugation. Reaction of the thiolated toxin with the maleimide-activated antibody creates the immunotoxin through thioether bond formation. Figure 21.13 Sulfo-SMCC may be used to activate antibody molecules for coupling to thiolated toxin components. An intact A-B toxin molecule can be modified to contain sulfhydryls by treatment with 2-iminothiolane. Thiolation with this reagent retains the cytotoxic properties of the toxin while generating a sulfhydryl for conjugation. Reaction of the thiolated toxin with the maleimide-activated antibody creates the immunotoxin through thioether bond formation.
See other pages where Toxin thiolation using 2-iminothiolane is mentioned: [Pg.524]    [Pg.504]    [Pg.830]    [Pg.837]    [Pg.840]    [Pg.848]    [Pg.520]    [Pg.529]    [Pg.537]    [Pg.500]    [Pg.509]    [Pg.517]   
See also in sourсe #XX -- [ Pg.852 ]



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