Imaging cellular fluorescent

Q. Ruan, Y. Chen, E. Gratton, M. Glaser, W.M. Mantulin, Cellular characterization of adenylate kinase and its isoform two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy, Biophys. J. 83, 3177-3187(2002)... 

Figure 9.4 Element array by SXFM. (A) Scheme of imaging cellular elements by SXFM. Coherent X-rays are focused on each area and the X-ray fluorescence from each element is detected. (B) SXFM analysis after cisplatin treatment. Cell morphologies are shown at xlOO magnifications (left). Each field of view is equivalent to an area of 70x70 pm. Results are shown for PC/SEN (top) and PC/RES cells (bottom). (C) Element array based on SXFM analysis. The mean signal intensity of each element obtained by SXFM analysis was calculated, and the fold increase of elements in PC/ RES cells was depicted by using the intensity in PC/SEN cells as a standard (left). A part of analyzed elements is shown. The fold increase of elements in PC/SEN and PC/RES cells after cisplatin treatment was also shown by using the intensity in PC/SEN before cisplatin treatment as a standard (right). Adapted with permission from Shimura et al. 2005 American Association For Cancer Research.

Day RN, Davidson MW (2009) The fluorescent protein palette tools for cellular imaging. Chem Soc Rev 38 2887-2921... 

Raluca Niesner, B. P., Schliische, P. and Gericke, K. H. (2004). Noniterative Biexponential fluorescence lifetime imaging in the investigation of cellular metabolism by means of NAD(P)H autofluorescence. Chem. Phys. Chem. J, 1141-9. 

The immobilisation of proteins into inorganic mesoporous host materials has attracted considerable attention due to the potential applications in biochemical, biomedical, industrial and bio-analytical fields [1] Biocompatible supports endowed with fluorescent tracers and adequately modified for specific interactions with cellular antigens are an amenable tool for image in living cells processes that are relevant to diseases. 

The staining of germinated pollen of Hippeastrum hybridum with colchicine demonstrates green-yellow emission of microtubules (better vision in black-white image) around nuclei of pollen grain (threads at the division of the nucleus) and spermium on the tip of the pollen tube, where spermium moves, as well as in some bridge sites of the tube (Fig. 10). The similar fluorescent allelochemicals may be also used as fluorescent dyes at the cellular diagnostics (Roshchina, 2005 b). 

Squaraine rotaxane dyes also were utilized as extremely bright and highly stable NIR fluorescent probes for in vitro and in vivo optical imaging of live and fixed cells [55]. These probes were modified for targeting of different cellular locations, namely,... 

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