Image transient fluorescence

Figure 14. Confocal fluorescence images of single molecules of (a) the model compound 69 and (c) the multichromophoric dendri-mer 70 in a polyvinylbutyral (PVB) film. The fluorescence intensity transients (fluorescence intensity versus time) exhibit typical on-off behavior for 69 (b) and jumps between different emissive levels for 70 (d). Pictures taken from ref. [48],...

Fig. 10.7. Yeast expressing (RCZy) or lackingyeastRCZTi (rceiA) were transiently treated (1 h) with DMSO, NSC294526 (11.1 pM) or NSC321329 (10.8 pM), indneed to express GFP-Ras2p for 6 h, and imaged by fluorescence microscopy. The strains all lack STE24 (i.e., ste24A).

Transient Fluorescence Image with IR Super-Resolution in Solution... 

To confirm that the transient fluorescence is indeed an action signal due to resonant infrared absorption, the fluorescence intensity in the image was measured as a function of IR wavelength (TFD-IR spectroscopy, [27]). Figure 29.4e shows the TFD-IR spectrum of Rhodamine-6G. When the IR wavelength was scanned over the... 

Figure 29.5 Picosecond time-resolved transient fluorescence images at several delay times, when the IR (diameter, 2.5 mm0) and visible (diameter, 7 mm()) lights are fixed to 3400nm and 621 nm, respectively.

Lukins, P.B., Rehman, S., Stevens, G.B. and George, D. (2005) Time-resolved spectroscopic fluorescence imaging, transient absorption and vibrational... 

Gonczy This is very difficult to quantitate. As you have seen from the immunofluorescence images, there are many microtubules on either side in wild-type moreover, anaphase B takes places within a couple of minutes. Therefore, it will be difficult to uncover potential transient changes in microtubule numbers using fixed specimens. However, we have generated a green fluorescent protein... 

Holub, O., Seufferheld, M. J., Gohlke, C., Govindjee, G. J., Heiss, G. J. and Clegg, R. M. (2007). Fluorescence lifetime imaging microscopy of Chlamydomonas reinhardtii Non-photochemical quenching mutants and the effect of photosynthetic inhibitors on the slow chlorophyll fluorescence transients. J. Microsc. 226, 90-120. 

Traditional optical spectroscopy, such as fluorescence imaging, often gets a substantial boost from nonlinear optics, which, as demonstrated by Fujino and Tahara (Chapter 3), can provide temporal resolution and the ability to detect transient chemical species. 

Figure 1. Diagram of apparatus for picosecond fluorescence studies using streak camera detection. A laser oscillator B dye cell C output reflector D polarizer E spark gap F KDP pockels cell G polarizer (crossed with D) H clear glass beamsplitter J laser amplifier K pin photodiode L transient digitizer M,N 1054 nm reflectors P 2nd harmonic generator Q 3rd or 4th harmonic generator R spectrograph S streak camera T biplanar photodiode U image...

XPS (x-ray photoelectron spectroscopy) utilizes photoionization and energy-disperse analysis of the emitted photoelectrons to study the composition and electronic state of a region of the surface of a zeolite. However, aU these techniques are destructive ones, and for that reason other methods such as isotopic-transient experiments or reflectance [16] and fluorescence [17] imaging can be used to estimate the effective membrane thickness. 

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