Kohler illumination

For the examination of wet mounts, the light of the microscope must be properly adjusted. To achieve optimal resolution, the condenser should be centered and focused for Kohler illumination (racked up). To achieve contrast of the objects in the field, light intensity is diminished with the iris diaphragm of the condenser rather than by lowering the condenser. 

It is essential to use Kohler illumination, which is a method for adjusting the illumination pathway to achieve maximum resolution and minimum chromatic error. The information is in the documentation for your... 

The shape of the modulation vs. frequency curve for a given NA and wavelength is dependent on the degree of spatial coherency illuminating system as shown in Figure 18. Two types of illumination systems are used in projection printers, Kohler illumination and critical illumination, shown in Figures 19a and b respectively. With Kohler illumination. 

Figure 19. Two common types of illumination systems (a) Kohler, (b)...

Figure 1. Crystals of calcium phosphate (arrows) in an unstained smear of a cheese sauce product. A small sample of the product was placed on a slide and observed by Kohler Illumination. If quantification is necessary, sample preparation is easily standardized to allow for particulate enumeration, (x 80)...

Figure 7. The left hand side shows an image of comminuted Process Cheese Spread stained for sulfate groups by application of Alcian blue at pH 1.0. Carrageenan is localized as the darker staining material in the micrograph. The right hand side of the same Figure shows a similar cheese particle not stained with Alcian Blue. Kohler illumination, (x 750)...

Tanaka Y, Bond MR, Kohler JJ (2008) Photocrosslinkers illuminate interactions in living cells. Mol Biosyst 4 473 -80... 

Kohler illumination should be used [11] to give uniform illumination of the viewing plane. Using an oil immersion objective, it is possible to resolve down to about 1 pm, although a 15% oversizing is to be expected at this level due to diffraction effects. 

The microscope should be set up using Kohler illumination monochromatic illumination produces a better image if small particles are to be measured. 

Kohler Illumination In microscopy, the illumination provided through... 

The illumination system comprises a series of relay optics and uniformizing optics that project the radiation from the source through the photomask to the entrance pupil of the projection optics of the lithographic lens, where it forms an image of the illumination source (see Fig. 13.8). This type of illumination system is referred to as Kohler illumination. The fraction of the pupil filled by the illuminator source s image determines the degree of coherence in the projection... 

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