Iduronic Acid 2-sulphate Sulphatase

Chapter 9. A substrate used in the assay of L-iduronic acid 2-sulphate sulphatase was obtained from sodium heparinate by methanolysis followed by nitrosation. Disaccharide monosulphates presumed to contain D-(methyl a-L-idopyranosiduronate-2-sulphate)-(l 4)-2,5-anhydro-D-mannose were isolated by gel filtration. Brucine L-guluronate and o-mannuronate, on the other hand, were obtained by treatment with brucine of the barium salts of the acids formed on hydrolysis of alginic add. ... 

Sodium heparinate has been treated sequentially with methanolic hydrogen chloride and nitrous acid to produce 0-(methyl a-L-idopyranosyluronate 2-sulphate-(l -> 4)-2,5-anhydro-D-mannose. This disaccharide can be used for direct measurement of L-iduronic acid 2-sulphate sulphatase, an enzyme which is defective in Hunter s syndrome (mucopolysaccharidosis II). 

Glycopeptide-linkage hydrolases a-L-Guluronan lyase Heparin hydrolase Hyaluronate lyase Hyaluronidase L-Iduronic acid 2-sulphate sulphatase a-L-Iduronidase Inulinase Isoamylase Isopullulanase a-Lactalbumin Lactose synthase... 

L-Iduronic Acid 2-Sulphate Sulphatases.—2,5-Anhydro-4-0-(a-L-idopyranuronosyl 2-sulphate)-D-[l- H]mannitol 6-sulphate has been recommended as a substrate for the measurement of L-iduronic acid 2-sulphate sulphatase activity, since it furnishes a radioactive monosulphate that is readily separated by paper chromatography or electrophoresis. The enzymic reaction followed Michaelis-Menten kinetics 3 mmol 1 ) and was strongly inhibited by phosphate ions. 

L-Iduronic Acid 2-SuIphate Sulphatases. — L-Iduronic acid sulphate sulphatase activity has been recognized in rat skin (see pp. 533-536). ... 

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