Hypoxia-regulated signal transduction pathways

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We next evaluated the effect of hypoxia on the p42/p44 MAPK pathway in PC12 cells (10). Western blot analysis showed that exposure to 5% O2 for 6hr induced a marked increase in tyrosine phosphorylation of both p42 and p44 MAPK, while the total MAPK was unchanged by hypoxia (Fig. 4). The phosphorylation of p42 and p44 by hypoxia was somewhat less than that measured when PC12 cells 

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