Hypersensitivity reactions modification

In type I hypersensitivity reactions, the penicillin RAST has been used by several authors to estimate the specific IgE response (Assem 1972 Wide and Juhlin 1971). Using a modification of the classical RAST which improves the sensivity of the tests, Adkinson (1975) found an excellent correlation between the positive clinical history, skin tests with PPL, and the results of the RAST. 

Several in vivo methods exist and are used as tools to determine the potential for contact, and potentially respiratory, hypersensitivity (e.g., the local lymph node (LENA) (Kimber et al., 2002) and popliteal lymph node (PLNA) (Pieters, 2001) assays) and the mouse drug allergy model, which is a modification of the LENA, demonstrates promise as a screening tool in that a number of drugs which cause idiosyncratic hypersensitivity reactions in humans were positive in this assay (Whritenour et al., 2014 Zhu et al., 2014). However, there are no fully predictive animal models of drug allergy in humans. 

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